5EXH

Crystal structure of mTET3-CXXC domain in complex with 5-carboxylcytosine DNA at 1.3 Angstroms resolution.

5EXH の概要

• エントリーDOI: 10.2210/pdb5exh/pdb
• 分子名称: DNA (5'-D(*GP*AP*AP*TP*CP*(1CC)P*GP*GP*AP*TP*TP*C)-3'), Methylcytosine dioxygenase TET3, ZINC ION, ... (4 entities in total)
• 機能のキーワード: mouse tet3, complex, 5-carboxylcytosine, reader, oxidoreductase-dna complex, oxidoreductase/dna
• 由来する生物種: Mus musculus (Mouse); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 13045.29

構造登録者

Song, J. (登録日: 2015-11-23, 公開日: 2016-02-03, 最終更新日: 2023-09-27)

主引用文献

Jin, S.G.,Zhang, Z.M.,Dunwell, T.L.,Harter, M.R.,Wu, X.,Johnson, J.,Li, Z.,Liu, J.,Szabo, P.E.,Lu, Q.,Xu, G.L.,Song, J.,Pfeifer, G.P.
Tet3 Reads 5-Carboxylcytosine through Its CXXC Domain and Is a Potential Guardian against Neurodegeneration.
Cell Rep, 14:493-505, 2016

PubMed Abstract: We report that the mammalian 5-methylcytosine (5mC) oxidase Tet3 exists as three major isoforms and characterized the full-length isoform containing an N-terminal CXXC domain (Tet3FL). This CXXC domain binds to unmethylated CpGs, but, unexpectedly, its highest affinity is toward 5-carboxylcytosine (5caC). We determined the crystal structure of the CXXC domain-5caC-DNA complex, revealing the structural basis of the binding specificity of this domain as a reader of CcaCG sequences. Mapping of Tet3FL in neuronal cells shows that Tet3FL is localized precisely at the transcription start sites (TSSs) of genes involved in lysosome function, mRNA processing, and key genes of the base excision repair pathway. Therefore, Tet3FL may function as a regulator of 5caC removal by base excision repair. Active removal of accumulating 5mC from the TSSs of genes coding for lysosomal proteins by Tet3FL in postmitotic neurons of the brain may be important for preventing neurodegenerative diseases.

PubMed: 26774490
DOI: 10.1016/j.celrep.2015.12.044

実験手法

X-RAY DIFFRACTION (1.3 Å)