5EQ3

Crystal structure of the SrpA adhesin from Streptococcus sanguinis with a sialyl galactose disaccharide bound

5EQ3 の概要

• エントリーDOI: 10.2210/pdb5eq3/pdb
• 関連するPDBエントリー: 5EQ2 5EQ3
• 分子名称: Platelet-binding glycoprotein, N-glycolyl-alpha-neuraminic acid-(2-3)-methyl beta-D-galactopyranoside, CALCIUM ION, ... (6 entities in total)
• 機能のキーワード: bacterial adhesin, lectin, immunoglobulin fold, serine-rich repeat, sugar binding protein
• 由来する生物種: Streptococcus sanguinis
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 45072.77

構造登録者

Loukachevitch, L.V.,McCulloch, K.M.,Vann, K.R.,Wawrzak, Z.,Anderson, S.,Iverson, T.M. (登録日: 2015-11-12, 公開日: 2016-01-27, 最終更新日: 2024-03-06)

主引用文献

Bensing, B.A.,Loukachevitch, L.V.,McCulloch, K.M.,Yu, H.,Vann, K.R.,Wawrzak, Z.,Anderson, S.,Chen, X.,Sullam, P.M.,Iverson, T.M.
Structural Basis for Sialoglycan Binding by the Streptococcus sanguinis SrpA Adhesin.
J.Biol.Chem., 291:7230-7240, 2016

PubMed Abstract: Streptococcus sanguinisis a leading cause of infective endocarditis, a life-threatening infection of the cardiovascular system. An important interaction in the pathogenesis of infective endocarditis is attachment of the organisms to host platelets.S. sanguinisexpresses a serine-rich repeat adhesin, SrpA, similar in sequence to platelet-binding adhesins associated with increased virulence in this disease. In this study, we determined the first crystal structure of the putative binding region of SrpA (SrpABR) both unliganded and in complex with a synthetic disaccharide ligand at 1.8 and 2.0 Å resolution, respectively. We identified a conserved Thr-Arg motif that orients the sialic acid moiety and is required for binding to platelet monolayers. Furthermore, we propose that sequence insertions in closely related family members contribute to the modulation of structural and functional properties, including the quaternary structure, the tertiary structure, and the ligand-binding site.

PubMed: 26833566
DOI: 10.1074/jbc.M115.701425

実験手法

X-RAY DIFFRACTION (2 Å)