5EO8
Crystal structure of AOL(868)
Summary for 5EO8
| Entry DOI | 10.2210/pdb5eo8/pdb |
| Related | 5EO7 |
| Descriptor | Predicted protein, methyl 1-seleno-beta-L-fucopyranoside (3 entities in total) |
| Functional Keywords | seleno-fucoses, phaing, lectin, aspergillus oryzae, sugar binding protein |
| Biological source | Aspergillus oryzae RIB40 (Yellow koji mold) |
| Total number of polymer chains | 1 |
| Total formula weight | 36022.50 |
| Authors | Kato, R.,Kiso, M.,Ishida, H.,Ando, H.,Suzuki, T.,Shimabukuro, S.,Makyio, H. (deposition date: 2015-11-10, release date: 2016-06-29, Last modification date: 2024-03-20) |
| Primary citation | Makyio, H.,Shimabukuro, J.,Suzuki, T.,Imamura, A.,Ishida, H.,Kiso, M.,Ando, H.,Kato, R. Six independent fucose-binding sites in the crystal structure of Aspergillus oryzae lectin Biochem.Biophys.Res.Commun., 477:477-482, 2016 Cited by PubMed Abstract: The crystal structure of AOL (a fucose-specific lectin of Aspergillus oryzae) has been solved by SAD (single-wavelength anomalous diffraction) and MAD (multi-wavelength anomalous diffraction) phasing of seleno-fucosides. The overall structure is a six-bladed β-propeller similar to that of other fucose-specific lectins. The fucose moieties of the seleno-fucosides are located in six fucose-binding sites. Although the Arg and Glu/Gln residues bound to the fucose moiety are common to all fucose-binding sites, the amino-acid residues involved in fucose binding at each site are not identical. The varying peak heights of the seleniums in the electron density map suggest that each fucose-binding site has a different carbohydrate binding affinity. PubMed: 27318092DOI: 10.1016/j.bbrc.2016.06.069 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.6 Å) |
Structure validation
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