5EO8

Crystal structure of AOL(868)

5EO8 の概要

• エントリーDOI: 10.2210/pdb5eo8/pdb
• 関連するPDBエントリー: 5EO7
• 分子名称: Predicted protein, methyl 1-seleno-beta-L-fucopyranoside (3 entities in total)
• 機能のキーワード: seleno-fucoses, phaing, lectin, aspergillus oryzae, sugar binding protein
• 由来する生物種: Aspergillus oryzae RIB40 (Yellow koji mold)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 36022.50

構造登録者

Kato, R.,Kiso, M.,Ishida, H.,Ando, H.,Suzuki, T.,Shimabukuro, S.,Makyio, H. (登録日: 2015-11-10, 公開日: 2016-06-29, 最終更新日: 2024-03-20)

主引用文献

Makyio, H.,Shimabukuro, J.,Suzuki, T.,Imamura, A.,Ishida, H.,Kiso, M.,Ando, H.,Kato, R.
Six independent fucose-binding sites in the crystal structure of Aspergillus oryzae lectin
Biochem.Biophys.Res.Commun., 477:477-482, 2016

PubMed Abstract: The crystal structure of AOL (a fucose-specific lectin of Aspergillus oryzae) has been solved by SAD (single-wavelength anomalous diffraction) and MAD (multi-wavelength anomalous diffraction) phasing of seleno-fucosides. The overall structure is a six-bladed β-propeller similar to that of other fucose-specific lectins. The fucose moieties of the seleno-fucosides are located in six fucose-binding sites. Although the Arg and Glu/Gln residues bound to the fucose moiety are common to all fucose-binding sites, the amino-acid residues involved in fucose binding at each site are not identical. The varying peak heights of the seleniums in the electron density map suggest that each fucose-binding site has a different carbohydrate binding affinity.

PubMed: 27318092
DOI: 10.1016/j.bbrc.2016.06.069

実験手法

X-RAY DIFFRACTION (1.6 Å)