5EN2

Molecular basis for antibody-mediated neutralization of New World hemorrhagic fever mammarenaviruses

5EN2 の概要

• エントリーDOI: 10.2210/pdb5en2/pdb
• 分子名称: GD01 heavy chain, GD01 light chain, Pre-glycoprotein polyprotein GP complex, ... (8 entities in total)
• 機能のキーワード: immunoglobulin, glycoprotein, virus, complex, viral protein-immune system complex, viral protein/immune system
• 由来する生物種: Mus musculus; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 65855.84

構造登録者

Mahmutovic, S.,Clark, L.,Levis, S.,Briggiler, A.,Enria, D.,Harrison, S.C.,Abraham, J. (登録日: 2015-11-09, 公開日: 2015-12-30, 最終更新日: 2024-11-20)

主引用文献

Mahmutovic, S.,Clark, L.,Levis, S.C.,Briggiler, A.M.,Enria, D.A.,Harrison, S.C.,Abraham, J.
Molecular Basis for Antibody-Mediated Neutralization of New World Hemorrhagic Fever Mammarenaviruses.
Cell Host Microbe, 18:705-713, 2015

PubMed Abstract: In the Western hemisphere, at least five mammarenaviruses cause human viral hemorrhagic fevers with high case fatality rates. Junín virus (JUNV) is the only hemorrhagic fever virus for which transfusion of survivor immune plasma that contains neutralizing antibodies ("passive immunity") is an established treatment. Here, we report the structure of the JUNV surface glycoprotein receptor-binding subunit (GP1) bound to a neutralizing monoclonal antibody. The antibody engages the GP1 site that binds transferrin receptor 1 (TfR1)-the host cell surface receptor for all New World hemorrhagic fever mammarenaviruses-and mimics an important receptor contact. We show that survivor immune plasma contains antibodies that bind the same epitope. We propose that viral receptor-binding site accessibility explains the success of passive immunity against JUNV and that this functionally conserved epitope is a potential target for therapeutics and vaccines to limit infection by all New World hemorrhagic fever mammarenaviruses.

PubMed: 26651946
DOI: 10.1016/j.chom.2015.11.005

実験手法

X-RAY DIFFRACTION (1.821 Å)