5EM1

Crystal structure of ragweed allergen Amb a 8

5EM1 の概要

• エントリーDOI: 10.2210/pdb5em1/pdb
• 関連するPDBエントリー: 5EM0
• 分子名称: Profilin, BENZOIC ACID, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: allergen
• 由来する生物種: Ambrosia artemisiifolia (Short ragweed)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14750.26

構造登録者

Offermann, L.R.,He, J.Z.,Perdue, M.L.,Chruszcz, M. (登録日: 2015-11-05, 公開日: 2016-06-08, 最終更新日: 2023-09-27)

主引用文献

Offermann, L.R.,Schlachter, C.R.,Perdue, M.L.,Majorek, K.A.,He, J.Z.,Booth, W.T.,Garrett, J.,Kowal, K.,Chruszcz, M.
Structural, Functional, and Immunological Characterization of Profilin Panallergens Amb a 8, Art v 4, and Bet v 2.
J.Biol.Chem., 291:15447-15459, 2016

PubMed Abstract: Ragweed allergens affect several million people in the United States and Canada. To date, only two ragweed allergens, Amb t 5 and Amb a 11, have their structures determined and deposited to the Protein Data Bank. Here, we present structures of methylated ragweed allergen Amb a 8, Amb a 8 in the presence of poly(l-proline), and Art v 4 (mugwort allergen). Amb a 8 and Art v 4 are panallergens belonging to the profilin family of proteins. They share significant sequence and structural similarities, which results in cross-recognition by IgE antibodies. Molecular and immunological properties of Amb a 8 and Art v 4 are compared with those of Bet v 2 (birch pollen allergen) as well as with other allergenic profilins. We purified recombinant allergens that are recognized by patient IgE and are highly cross-reactive. It was determined that the analyzed allergens are relatively unstable. Structures of Amb a 8 in complex with poly(l-proline)10 or poly(l-proline)14 are the first structures of the plant profilin in complex with proline-rich peptides. Amb a 8 binds the poly(l-proline) in a mode similar to that observed in human, mouse, and P. falciparum profilin·peptide complexes. However, only some of the residues that form the peptide binding site are conserved.

PubMed: 27231348
DOI: 10.1074/jbc.M116.733659

実験手法

X-RAY DIFFRACTION (1.45 Å)