5EHF

Laccase from Antrodiella faginea

「5DAO」から置き換えられました

5EHF の概要

• エントリーDOI: 10.2210/pdb5ehf/pdb
• 分子名称: Laccase, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (7 entities in total)
• 機能のキーワード: oxidoreductase
• 由来する生物種: Antrodiella faginea
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 55570.26

構造登録者

Polyakov, K.M.,Glazunova, O.A.,Fedorova, T.V.,Dorovatovskii, P.V.,Koroleva, O.V. (登録日: 2015-10-28, 公開日: 2015-12-23, 最終更新日: 2024-10-23)

主引用文献

Glazunova, O.A.,Polyakov, K.M.,Moiseenko, K.V.,Kurzeev, S.A.,Fedorova, T.V.
Structure-function study of two new middle-redox potential laccases from basidiomycetes Antrodiella faginea and Steccherinum murashkinskyi.
Int. J. Biol. Macromol., 118:406-418, 2018

PubMed Abstract: Laccases are multicopper oxidases that catalyze oxidation of a wide range of organic and inorganic substrates accompanied by the reduction of dioxygen to water. The physicochemical and catalytic properties of two new fungal laccases from basidiomycetes Antrodiella faginea (AfL) and Steccherinum murashkinskyi (SmL) with middle redox potential of the T1 copper site were studied. The X-ray structures of AfL and SmL were solved at 1.75 Å and 0.95 Å, respectively. The oxidized state of copper ions in the active site was observed in AfL structure, while the mixture of oxidized and reduced states was observed in SmL structure. These oxidized and reduced states relate to the position of copper ions, their coordination, and nature and position of oxygen ligands. Comparative analysis of the T1 site environment of laccases with known structure allowed us to highlight the six types of the secondary coordination sphere of the T1 copper. The solvent accessible surface area of the conservative region of the secondary coordination sphere of the T1 copper correlates with its the redox potential. It was shown that the laccase classification by the structure of the T1 copper secondary coordination sphere is in agreement to ecophysiological behavior of laccase producing fungi.

PubMed: 29890251
DOI: 10.1016/j.ijbiomac.2018.06.038

実験手法

X-RAY DIFFRACTION (1.75 Å)