5EAU

5-EPI-ARISTOLOCHENE SYNTHASE FROM NICOTIANA TABACUM

5EAU の概要

• エントリーDOI: 10.2210/pdb5eau/pdb
• 分子名称: 5-EPI-ARISTOLOCHENE SYNTHASE, MAGNESIUM ION, TRIFLUOROFURNESYL DIPHOSPHATE, ... (4 entities in total)
• 機能のキーワード: isoprenoid synthase, 5-epi-aristolochene synthase, natural products biosynthesis, isoprenoid cyclase
• 由来する生物種: Nicotiana tabacum (common tobacco)
• 細胞内の位置: Cytoplasm: Q40577
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 63528.39

構造登録者

Starks, C.M.,Back, K.,Chappell, J.,Noel, J.P. (登録日: 1997-12-11, 公開日: 1998-04-08, 最終更新日: 2024-05-22)

主引用文献

Starks, C.M.,Back, K.,Chappell, J.,Noel, J.P.
Structural basis for cyclic terpene biosynthesis by tobacco 5-epi-aristolochene synthase.
Science, 277:1815-1820, 1997

PubMed Abstract: Terpene cyclases catalyze the synthesis of cyclic terpenes with 10-, 15-, and 20-carbon acyclic isoprenoid diphosphates as substrates. Plants have been a source of these natural products by providing a homologous set of terpene synthases. The crystal structures of 5-epi-aristolochene synthase, a sesquiterpene cyclase from tobacco, alone and complexed separately with two farnesyl diphosphate analogs were analyzed. These structures reveal an unexpected enzymatic mechanism for the synthesis of the bicyclic product, 5-epi-aristolochene, and provide a basis for understanding the stereochemical selectivity displayed by other cyclases in the biosynthesis of pharmacologically important cyclic terpenes. As such, these structures provide templates for the engineering of novel terpene cyclases.

PubMed: 9295271
DOI: 10.1126/science.277.5333.1815

実験手法

X-RAY DIFFRACTION (2.15 Å)