5E9P

Spirochaeta thermophila X module - CBM64 - wildtype

5E9P の概要

• エントリーDOI: 10.2210/pdb5e9p/pdb
• 分子名称: Cellulase, glycosyl hydrolase family 5, TPS linker, domain X, MALONATE ION (3 entities in total)
• 機能のキーワード: carbohydrate-binding module 64, cbm64, cellulose and xylan binding, type a cbm, jelly roll, hydrolase
• 由来する生物種: Spirochaeta thermophila
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 10127.80

構造登録者

Schiefner, A.,Skerra, A. (登録日: 2015-10-15, 公開日: 2016-03-02, 最終更新日: 2024-01-10)

主引用文献

Schiefner, A.,Angelov, A.,Liebl, W.,Skerra, A.
Structural basis for cellulose binding by the type A carbohydrate-binding module 64 of Spirochaeta thermophila.
Proteins, 84:855-858, 2016

PubMed Abstract: Spirochaeta thermophila secretes seven glycoside hydrolases for plant biomass degradation that carry a carbohydrate-binding module 64 (CBM64) appended at the C-terminus. CBM64 adsorbs to various β1-4-linked pyranose substrates and shows high affinity for cellulose. We present the first crystal structure of a CBM64 at 1.2 Å resolution, which reveals a jelly-roll-like fold corresponding to a surface-binding type A CBM. Modeling of its interaction with cellulose indicates that CBM64 achieves association with the hydrophobic face of β-linked pyranose chains via a unique coplanar arrangement of four exposed tryptophan side chains. Proteins 2016; 84:855-858. © 2016 Wiley Periodicals, Inc.

PubMed: 26868291
DOI: 10.1002/prot.25010

実験手法

X-RAY DIFFRACTION (1.2 Å)