5E9H

Structural insights of isocitrate lyases from Fusarium graminearum

5E9H の概要

• エントリーDOI: 10.2210/pdb5e9h/pdb
• 関連するPDBエントリー: 5E9F 5E9G
• 分子名称: Isocitrate lyase, MANGANESE (II) ION, MALONATE ION, ... (4 entities in total)
• 機能のキーワード: tim beta/alpha-barrel, lyase activity, lyase
• 由来する生物種: Gibberella zeae PH-1 (Wheat head blight fungus)
• 細胞内の位置: Glyoxysome : Q4HYR2
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 124100.86

構造登録者

Park, Y.,Cho, Y.,Lee, Y.-H.,Lee, Y.-W.,Rhee, S. (登録日: 2015-10-15, 公開日: 2016-04-27, 最終更新日: 2023-11-08)

主引用文献

Park, Y.,Cho, Y.,Lee, Y.-H.,Lee, Y.-W.,Rhee, S.
Crystal structure and functional analysis of isocitrate lyases from Magnaporthe oryzae and Fusarium graminearum
J.Struct.Biol., 194:395-403, 2016

PubMed Abstract: The glyoxylate cycle bypasses a CO2-generating step in the tricarboxylic acid (TCA) cycle and efficiently assimilates C2 compounds into intermediates that can be used in later steps of the TCA cycle. It plays an essential role in pathogen survival during host infection such that the enzymes involved in this cycle have been suggested as potential drug targets against human pathogens. Isocitrate lyase (ICL) catalyzes the first-step reaction of the glyoxylate cycle, using isocitrate from the TCA cycle as the substrate to produce succinate and glyoxylate. In this study we report the crystal structure of Magnaporthe oryzae ICL in both the ligand-free form and as a complex with Mg(2+), glyoxylate, and glycerol, as well as the structure of the Fusarium graminearum ICL complexed with Mn(2+) and malonate. We also describe the ligand-induced conformational changes in the catalytic loop and C-terminal region, both of which are essential for catalysis. Using various mutant ICLs in an activity assay, we gained insight into the function of residues within the active site. These structural and functional analyses provide detailed information with regard to fungal ICLs.

PubMed: 27016285
DOI: 10.1016/j.jsb.2016.03.019

実験手法

X-RAY DIFFRACTION (2.3 Å)