5E37

Redox protein from Chlamydomonas reinhardtii

5E37 の概要

• エントリーDOI: 10.2210/pdb5e37/pdb
• 分子名称: EF-Hand domain-containing thioredoxin, CALCIUM ION (3 entities in total)
• 機能のキーワード: calredoxin, calcium-, redox-, chlamydomonas reinhardtii, oxidoreductase
• 由来する生物種: Chlamydomonas reinhardtii
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 80221.42

構造登録者

Charoenwattansatien, R.,Hochmal, A.K.,Zinzius, K.,Muto, R.,Tanaka, H.,Hippler, M.,Kurisu, G. (登録日: 2015-10-02, 公開日: 2016-06-22, 最終更新日: 2024-10-23)

主引用文献

Hochmal, A.K.,Zinzius, K.,Charoenwattanasatien, R.,Gabelein, P.,Mutoh, R.,Tanaka, H.,Schulze, S.,Liu, G.,Scholz, M.,Nordhues, A.,Offenborn, J.N.,Petroutsos, D.,Finazzi, G.,Fufezan, C.,Huang, K.,Kurisu, G.,Hippler, M.
Calredoxin represents a novel type of calcium-dependent sensor-responder connected to redox regulation in the chloroplast
Nat Commun, 7:11847-11847, 2016

PubMed Abstract: Calcium (Ca(2+)) and redox signalling play important roles in acclimation processes from archaea to eukaryotic organisms. Herein we characterized a unique protein from Chlamydomonas reinhardtii that has the competence to integrate Ca(2+)- and redox-related signalling. This protein, designated as calredoxin (CRX), combines four Ca(2+)-binding EF-hands and a thioredoxin (TRX) domain. A crystal structure of CRX, at 1.6 Å resolution, revealed an unusual calmodulin-fold of the Ca(2+)-binding EF-hands, which is functionally linked via an inter-domain communication path with the enzymatically active TRX domain. CRX is chloroplast-localized and interacted with a chloroplast 2-Cys peroxiredoxin (PRX1). Ca(2+)-binding to CRX is critical for its TRX activity and for efficient binding and reduction of PRX1. Thereby, CRX represents a new class of Ca(2+)-dependent 'sensor-responder' proteins. Genetically engineered Chlamydomonas strains with strongly diminished amounts of CRX revealed altered photosynthetic electron transfer and were affected in oxidative stress response underpinning a function of CRX in stress acclimation.

PubMed: 27297041
DOI: 10.1038/ncomms11847

実験手法

X-RAY DIFFRACTION (1.6 Å)