5DWC

Crystal structure of restriction endonuclease AgeI

5DWC の概要

• エントリーDOI: 10.2210/pdb5dwc/pdb
• 分子名称: Type-2 restriction enzyme AgeI (2 entities in total)
• 機能のキーワード: restriction endonuclease, pd-(d/e)xk nuclease, hydrolase
• 由来する生物種: Thalassobius gelatinovorus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 30593.52

構造登録者

Tamulaitiene, G.,Ramonaite, I.,Grazulis, S.,Siksnys, V. (登録日: 2015-09-22, 公開日: 2016-09-28, 最終更新日: 2024-11-13)

主引用文献

Tamulaitiene, G.,Jovaisaite, V.,Tamulaitis, G.,Songailiene, I.,Manakova, E.,Zaremba, M.,Grazulis, S.,Xu, S.Y.,Siksnys, V.
Restriction endonuclease AgeI is a monomer which dimerizes to cleave DNA.
Nucleic Acids Res., 45:3547-3558, 2017

PubMed Abstract: Although all Type II restriction endonucleases catalyze phosphodiester bond hydrolysis within or close to their DNA target sites, they form different oligomeric assemblies ranging from monomers, dimers, tetramers to higher order oligomers to generate a double strand break in DNA. Type IIP restriction endonuclease AgeI recognizes a palindromic sequence 5΄-A/CCGGT-3΄ and cuts it ('/' denotes the cleavage site) producing staggered DNA ends. Here, we present crystal structures of AgeI in apo and DNA-bound forms. The structure of AgeI is similar to the restriction enzymes that share in their target sites a conserved CCGG tetranucleotide and a cleavage pattern. Structure analysis and biochemical data indicate, that AgeI is a monomer in the apo-form both in the crystal and in solution, however, it binds and cleaves the palindromic target site as a dimer. DNA cleavage mechanism of AgeI is novel among Type IIP restriction endonucleases.

PubMed: 28039325
DOI: 10.1093/nar/gkw1310

実験手法

X-RAY DIFFRACTION (2.5 Å)