5DVS

Crystal Structure of the ER-alpha Ligand-binding Domain in Complex with a 2-Methyl-substituted Triaryl-imine 4,4'-[(2-methylphenyl)carbonimidoyl]diphenol

5DVS の概要

• エントリーDOI: 10.2210/pdb5dvs/pdb
• 分子名称: Estrogen receptor, Nuclear receptor coactivator 2, 4,4'-[(2-methylphenyl)carbonimidoyl]diphenol, ... (4 entities in total)
• 機能のキーワード: nuclear receptor, transcription factor, ligand binding, protein-ligand complex, transcription
• 由来する生物種: Homo sapiens (Human); 詳細
• 細胞内の位置: Isoform 1: Nucleus . Isoform 3: Nucleus. Nucleus: P03372; Nucleus: Q15596
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 62539.66

構造登録者

Nwachukwu, J.C.,Wright, N.J.,Srinivasan, S.,Zheng, Y.,Wang, S.,Min, J.,Dong, C.,Liao, Z.,Cavett, V.,Nowak, J.,Houtman, R.,Carlson, K.E.,Josan, J.S.,Elemento, O.,Katzenellenbogen, J.A.,Zhou, H.B.,Nettles, K.W. (登録日: 2015-09-21, 公開日: 2016-05-04, 最終更新日: 2024-03-06)

主引用文献

Nwachukwu, J.C.,Srinivasan, S.,Zheng, Y.,Wang, S.,Min, J.,Dong, C.,Liao, Z.,Nowak, J.,Wright, N.J.,Houtman, R.,Carlson, K.E.,Josan, J.S.,Elemento, O.,Katzenellenbogen, J.A.,Zhou, H.B.,Nettles, K.W.
Predictive features of ligand-specific signaling through the estrogen receptor.
Mol.Syst.Biol., 12:864-864, 2016

PubMed Abstract: Some estrogen receptor-α (ERα)-targeted breast cancer therapies such as tamoxifen have tissue-selective or cell-specific activities, while others have similar activities in different cell types. To identify biophysical determinants of cell-specific signaling and breast cancer cell proliferation, we synthesized 241 ERα ligands based on 19 chemical scaffolds, and compared ligand response using quantitative bioassays for canonical ERα activities and X-ray crystallography. Ligands that regulate the dynamics and stability of the coactivator-binding site in the C-terminal ligand-binding domain, called activation function-2 (AF-2), showed similar activity profiles in different cell types. Such ligands induced breast cancer cell proliferation in a manner that was predicted by the canonical recruitment of the coactivators NCOA1/2/3 and induction of the GREB1 proliferative gene. For some ligand series, a single inter-atomic distance in the ligand-binding domain predicted their proliferative effects. In contrast, the N-terminal coactivator-binding site, activation function-1 (AF-1), determined cell-specific signaling induced by ligands that used alternate mechanisms to control cell proliferation. Thus, incorporating systems structural analyses with quantitative chemical biology reveals how ligands can achieve distinct allosteric signaling outcomes through ERα.

PubMed: 27107013
DOI: 10.15252/msb.20156701

実験手法

X-RAY DIFFRACTION (2.28 Å)