5DSP

Structure of CO2 released apo-form of human carbonic anhydrase II with 40 sec warming

5DSP の概要

• エントリーDOI: 10.2210/pdb5dsp/pdb
• 関連するPDBエントリー: 5DSI 5DSJ 5DSK 5DSL 5DSM 5DSN 5DSO 5DSQ 5DSR
• 分子名称: Carbonic anhydrase 2, GLYCEROL (3 entities in total)
• 機能のキーワード: carbonic anhydrase ii, co2 release, intermediate states, lyase
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 29381.16

構造登録者

Kim, C.U.,Park, S.Y.,McKenna, R. (登録日: 2015-09-17, 公開日: 2016-05-04, 最終更新日: 2023-11-08)

主引用文献

Kim, C.U.,Song, H.,Avvaru, B.S.,Gruner, S.M.,Park, S.,McKenna, R.
Tracking solvent and protein movement during CO2 release in carbonic anhydrase II crystals
Proc. Natl. Acad. Sci. U.S.A., 113:5257-5262, 2016

PubMed Abstract: Carbonic anhydrases are mostly zinc metalloenzymes that catalyze the reversible hydration/dehydration of CO2/HCO3 (-) Previously, the X-ray crystal structures of CO2-bound holo (zinc-bound) and apo (zinc-free) human carbonic anhydrase IIs (hCA IIs) were captured at high resolution. Here, we present sequential timeframe structures of holo- [T = 0 s (CO2-bound), 50 s, 3 min, 10 min, 25 min, and 1 h] and apo-hCA IIs [T = 0 s, 50 s, 3 min, and 10 min] during the "slow" release of CO2 Two active site waters, WDW (deep water) and WDW' (this study), replace the vacated space created on CO2 release, and another water, WI (intermediate water), is seen to translocate to the proton wire position W1. In addition, on the rim of the active site pocket, a water W2' (this study), in close proximity to residue His64 and W2, gradually exits the active site, whereas His64 concurrently rotates from pointing away ("out") to pointing toward ("in") active site rotameric conformation. This study provides for the first time, to our knowledge, structural "snapshots" of hCA II intermediate states during the formation of the His64-mediated proton wire that is induced as CO2 is released. Comparison of the holo- and apo-hCA II structures shows that the solvent network rearrangements require the presence of the zinc ion.

PubMed: 27114542
DOI: 10.1073/pnas.1520786113

実験手法

X-RAY DIFFRACTION (1.4 Å)