5DPN

Engineered CBM X-2 L110F in complex with branched carbohydrate XXXG.

5DPN の概要

• エントリーDOI: 10.2210/pdb5dpn/pdb
• 分子名称: Xylanase, alpha-D-xylopyranose-(1-6)-beta-D-glucopyranose-(1-4)-[alpha-D-xylopyranose-(1-6)]beta-D-glucopyranose-(1-4)-[alpha-D-xylopyranose-(1-6)]beta-D-glucopyranose-(1-4)-beta-D-glucopyranose, CALCIUM ION, ... (4 entities in total)
• 機能のキーワード: carbohydrate binding module hydrogen bond h/d exchanged, sugar binding protein
• 由来する生物種: Rhodothermus marinus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 19042.74

構造登録者

Ohlin, M. (登録日: 2015-09-13, 公開日: 2015-10-28, 最終更新日: 2024-05-01)

主引用文献

Fisher, S.Z.,von Schantz, L.,Hakansson, M.,Logan, D.T.,Ohlin, M.
Neutron Crystallographic Studies Reveal Hydrogen Bond and Water-Mediated Interactions between a Carbohydrate-Binding Module and Its Bound Carbohydrate Ligand.
Biochemistry, 54:6435-6438, 2015

PubMed Abstract: Carbohydrate-binding modules (CBMs) are key components of many carbohydrate-modifying enzymes. CBMs affect the activity of these enzymes by modulating bonding and catalysis. To further characterize and study CBM-ligand binding interactions, neutron crystallographic studies of an engineered family 4-type CBM in complex with a branched xyloglucan ligand were conducted. The first neutron crystal structure of a CBM-ligand complex reported here shows numerous atomic details of hydrogen bonding and water-mediated interactions and reveals the charged state of key binding cleft amino acid side chains.

PubMed: 26451738
DOI: 10.1021/acs.biochem.5b01058

実験手法

NEUTRON DIFFRACTION (1.606 Å)
X-RAY DIFFRACTION (1.6 Å)