5DO4

Thrombin-RNA aptamer complex

5DO4 の概要

• エントリーDOI: 10.2210/pdb5do4/pdb
• 関連するBIRD辞書のPRD_ID: PRD_000020
• 分子名称: Thrombin light chain, MAGNESIUM ION, Thrombin heavy chain, ... (11 entities in total)
• 機能のキーワード: thrombin-rna aptamer, phosphorodithioate rna, ps2-rna, ps2 linkage, hydrolase-rna complex, hydrolase/rna
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 43284.77

構造登録者

Pallan, P.S.,Egli, M. (登録日: 2015-09-10, 公開日: 2016-09-14, 最終更新日: 2024-10-30)

主引用文献

Abeydeera, N.D.,Egli, M.,Cox, N.,Mercier, K.,Conde, J.N.,Pallan, P.S.,Mizurini, D.M.,Sierant, M.,Hibti, F.E.,Hassell, T.,Wang, T.,Liu, F.W.,Liu, H.M.,Martinez, C.,Sood, A.K.,Lybrand, T.P.,Frydman, C.,Monteiro, R.Q.,Gomer, R.H.,Nawrot, B.,Yang, X.
Evoking picomolar binding in RNA by a single phosphorodithioate linkage.
Nucleic Acids Res., 44:8052-8064, 2016

PubMed Abstract: RNA aptamers are synthetic oligonucleotide-based affinity molecules that utilize unique three-dimensional structures for their affinity and specificity to a target such as a protein. They hold the promise of numerous advantages over biologically produced antibodies; however, the binding affinity and specificity of RNA aptamers are often insufficient for successful implementation in diagnostic assays or as therapeutic agents. Strong binding affinity is important to improve the downstream applications. We report here the use of the phosphorodithioate (PS2) substitution on a single nucleotide of RNA aptamers to dramatically improve target binding affinity by ∼1000-fold (from nanomolar to picomolar). An X-ray co-crystal structure of the α-thrombin:PS2-aptamer complex reveals a localized induced-fit rearrangement of the PS2-containing nucleotide which leads to enhanced target interaction. High-level quantum mechanical calculations for model systems that mimic the PS2 moiety and phenylalanine demonstrate that an edge-on interaction between sulfur and the aromatic ring is quite favorable, and also confirm that the sulfur analogs are much more polarizable than the corresponding phosphates. This favorable interaction involving the sulfur atom is likely even more significant in the full aptamer-protein complexes than in the model systems.

PubMed: 27566147
DOI: 10.1093/nar/gkw725

実験手法

X-RAY DIFFRACTION (1.859 Å)