5DN2

Human NRP2 b1 domain in complex with the peptide corresponding to the C-terminus of VEGF-A

5DN2 の概要

• エントリーDOI: 10.2210/pdb5dn2/pdb
• 分子名称: Neuropilin-2, Vascular endothelial growth factor A, 1,4-DIETHYLENE DIOXIDE, ... (5 entities in total)
• 機能のキーワード: signaling protein, neuropilin-2, vegfa, angiogenesis, heparin, receptor
• 由来する生物種: Homo sapiens (Human); 詳細
• 細胞内の位置: Membrane ; Single-pass type I membrane protein . Isoform s9: Secreted : O60462; Secreted : P15692
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 81763.29

構造登録者

Tsai, Y.C.I.,Frankel, P.,Fotinou, C.,Rana, R.,Zachary, I.,Djordjevic, S. (登録日: 2015-09-09, 公開日: 2016-07-20, 最終更新日: 2024-10-16)

主引用文献

Tsai, Y.C.,Fotinou, C.,Rana, R.,Yelland, T.,Frankel, P.,Zachary, I.,Djordjevic, S.
Structural studies of neuropilin-2 reveal a zinc ion binding site remote from the vascular endothelial growth factor binding pocket.
Febs J., 283:1921-1934, 2016

PubMed Abstract: Neuropilin-2 is a transmembrane receptor involved in lymphangiogenesis and neuronal development. In adults, neuropilin-2 and its homologous protein neuropilin-1 have been implicated in cancers and infection. Molecular determinants of the ligand selectivity of neuropilins are poorly understood. We have identified and structurally characterized a zinc ion binding site on human neuropilin-2. The neuropilin-2-specific zinc ion binding site is located near the interface between domains b1 and b2 in the ectopic region of the protein, remote from the neuropilin binding site for its physiological ligand, i.e. vascular endothelial growth factor. We also present an X-ray crystal structure of the neuropilin-2 b1 domain in a complex with the C-terminal sub-domain of VEGF-A. Zn(2+) binding to neuropilin-2 destabilizes the protein structure but this effect was counteracted by heparin, suggesting that modifications by glycans and zinc in the extracellular matrix may affect functional neuropilin-2 ligand binding and signalling activity.

PubMed: 26991001
DOI: 10.1111/febs.13711

実験手法

X-RAY DIFFRACTION (1.95 Å)