5DJ5

Crystal structure of rice DWARF14 in complex with synthetic strigolactone GR24

5DJ5 の概要

• エントリーDOI: 10.2210/pdb5dj5/pdb
• 分子名称: Probable strigolactone esterase D14, (3E,3aR,8bS)-3-({[(2R)-4-methyl-5-oxo-2,5-dihydrofuran-2-yl]oxy}methylidene)-3,3a,4,8b-tetrahydro-2H-indeno[1,2-b]furan-2-one (3 entities in total)
• 機能のキーワード: alpha/beta, hydrolase
• 由来する生物種: Oryza sativa subsp. japonica (Rice)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 58803.48

構造登録者

Zhou, X.E.,Zhao, L.-H.,Yi, W.,Wu, Z.-S.,Liu, Y.,Kang, Y.,Hou, L.,de Waal, P.W.,Li, S.,Jiang, Y.,Melcher, K.,Xu, H.E. (登録日: 2015-09-01, 公開日: 2015-10-28, 最終更新日: 2023-09-27)

主引用文献

Zhao, L.H.,Zhou, X.E.,Yi, W.,Wu, Z.,Liu, Y.,Kang, Y.,Hou, L.,de Waal, P.W.,Li, S.,Jiang, Y.,Scaffidi, A.,Flematti, G.R.,Smith, S.M.,Lam, V.Q.,Griffin, P.R.,Wang, Y.,Li, J.,Melcher, K.,Xu, H.E.
Destabilization of strigolactone receptor DWARF14 by binding of ligand and E3-ligase signaling effector DWARF3.
Cell Res., 25:1219-1236, 2015

PubMed Abstract: Strigolactones (SLs) are endogenous hormones and exuded signaling molecules in plant responses to low levels of mineral nutrients. Key mediators of the SL signaling pathway in rice include the α/β-fold hydrolase DWARF 14 (D14) and the F-box component DWARF 3 (D3) of the ubiquitin ligase SCF(D3) that mediate ligand-dependent degradation of downstream signaling repressors. One perplexing feature is that D14 not only functions as the SL receptor but is also an active enzyme that slowly hydrolyzes diverse natural and synthetic SLs including GR24, preventing the crystallization of a binary complex of D14 with an intact SL as well as the ternary D14/SL/D3 complex. Here we overcome these barriers to derive a structural model of D14 bound to intact GR24 and identify the interface that is required for GR24-mediated D14-D3 interaction. The mode of GR24-mediated signaling, including ligand recognition, hydrolysis by D14, and ligand-mediated D14-D3 interaction, is conserved in structurally diverse SLs. More importantly, D14 is destabilized upon the binding of ligands and D3, thus revealing an unusual mechanism of SL recognition and signaling, in which the hormone, the receptor, and the downstream effectors are systematically destabilized during the signal transduction process.

PubMed: 26470846
DOI: 10.1038/cr.2015.122

実験手法

X-RAY DIFFRACTION (2.4 Å)