5DHD

Crystal structure of ChBD2 from Thermococcus kodakarensis KOD1

5DHD の概要

• エントリーDOI: 10.2210/pdb5dhd/pdb
• 関連するPDBエントリー: 5DHE
• 分子名称: Chitinase, SULFATE ION, 3,6,9,12,15,18,21,24,27,30,33,36,39-TRIDECAOXAHENTETRACONTANE-1,41-DIOL, ... (4 entities in total)
• 機能のキーワード: chitin, chitinase, chitin binding domain, hydrolase
• 由来する生物種: Thermococcus kodakarensis KOD1
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14931.64

構造登録者

Hibi, M.,Niwa, S.,Takeda, K.,Miki, K. (登録日: 2015-08-30, 公開日: 2016-02-10, 最終更新日: 2023-11-08)

主引用文献

Hanazono, Y.,Takeda, K.,Niwa, S.,Hibi, M.,Takahashi, N.,Kanai, T.,Atomi, H.,Miki, K.
Crystal structures of chitin binding domains of chitinase from Thermococcus kodakarensis KOD1
Febs Lett., 590:298-304, 2016

PubMed Abstract: Chitinase from T. kodakarensis (TkChiA) catalyzes the hydrolysis of chitin. The enzyme consists of two catalytic and three binding domains (ChBD1, ChBD2 and ChBD3). ChBD2 and ChBD3 can bind to not only chitin but also cellulose. In both domains, the intervals of the side chains of the three tryptophan residues, which are located on the molecular surface, correspond to twice the length of the lattice of the chitin. A binding model with crystalline chitin implies that the tryptophan residues and a glutamate residue interact with the hexose ring by CH-π interactions and the amide group by a hydrogen bond, respectively.

PubMed: 26823175
DOI: 10.1002/1873-3468.12055

実験手法

X-RAY DIFFRACTION (1.27 Å)