5DDW

Crystal structure of aminotransferase CrmG from Actinoalloteichus sp. WH1-2216-6 in complex with the PMP external aldimine adduct with Caerulomycin M

5DDW の概要

• エントリーDOI: 10.2210/pdb5ddw/pdb
• 関連するPDBエントリー: 5DDS 5DDU
• 分子名称: CrmG, [5-hydroxy-4-({(E)-[(4-hydroxy-2,2'-bipyridin-6-yl)methylidene]amino}methyl)-6-methylpyridin-3-yl]methyl dihydrogen phosphate, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: aminotransferase, caerulomycin m, caerulomycin biosynthesis, transferase
• 由来する生物種: Actinoalloteichus sp. WH1-2216-6
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 231059.95

構造登録者

Xu, J.,Feng, Z.,Liu, J. (登録日: 2015-08-25, 公開日: 2016-08-10, 最終更新日: 2024-03-20)

主引用文献

Zhu, Y.,Xu, J.,Mei, X.,Feng, Z.,Zhang, L.,Zhang, Q.,Zhang, G.,Zhu, W.,Liu, J.,Zhang, C.
Biochemical and Structural Insights into the Aminotransferase CrmG in Caerulomycin Biosynthesis
Acs Chem.Biol., 11:943-952, 2016

PubMed Abstract: Caerulomycin A (CRM A 1) belongs to a family of natural products containing a 2,2'-bipyridyl ring core structure and is currently under development as a potent novel immunosuppressive agent. Herein, we report the functional characterization, kinetic analysis, substrate specificity, and structure insights of an aminotransferase CrmG in 1 biosynthesis. The aminotransferase CrmG was confirmed to catalyze a key transamination reaction to convert an aldehyde group to an amino group in the 1 biosynthetic pathway, preferring l-glutamate and l-glutamine as the amino donor substrates. The crystal structures of CrmG in complex with the cofactor 5'-pyridoxal phosphate (PLP) or 5'-pyridoxamine phosphate (PMP) or the acceptor substrate were determined to adopt a canonical fold-type I of PLP-dependent enzymes with a unique small additional domain. The structure guided site-directed mutagenesis identified key amino acid residues for substrate binding and catalytic activities, thus providing insights into the transamination mechanism of CrmG.

PubMed: 26714051
DOI: 10.1021/acschembio.5b00984

実験手法

X-RAY DIFFRACTION (2.3 Å)