5DDH

Structure of HLA-A2:01 with the 12-mer peptide F12K

5DDH の概要

• エントリーDOI: 10.2210/pdb5ddh/pdb
• 関連するPDBエントリー: 5D9S
• 分子名称: HLA class I histocompatibility antigen, A-2 alpha chain, Beta-2-microglobulin, 12-mer peptide F12K, ... (5 entities in total)
• 機能のキーワード: mhc, immune system, peptide-protein complex
• 由来する生物種: Homo sapiens (Human); 詳細
• 細胞内の位置: Membrane; Single-pass type I membrane protein: P01892; Secreted : P61769
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 45056.06

構造登録者

Remesh, S.G.,Zajonc, D. (登録日: 2015-08-24, 公開日: 2016-07-27, 最終更新日: 2024-11-06)

主引用文献

McMurtrey, C.,Trolle, T.,Sansom, T.,Remesh, S.G.,Kaever, T.,Bardet, W.,Jackson, K.,McLeod, R.,Sette, A.,Nielsen, M.,Zajonc, D.M.,Blader, I.J.,Peters, B.,Hildebrand, W.
Toxoplasma gondii peptide ligands open the gate of the HLA class I binding groove.
Elife, 5:-, 2016

PubMed Abstract: HLA class I presentation of pathogen-derived peptide ligands is essential for CD8+ T-cell recognition of Toxoplasma gondii infected cells. Currently, little data exist pertaining to peptides that are presented after T. gondii infection. Herein we purify HLA-A*02:01 complexes from T. gondii infected cells and characterize the peptide ligands using LCMS. We identify 195 T. gondii encoded ligands originating from both secreted and cytoplasmic proteins. Surprisingly, T. gondii ligands are significantly longer than uninfected host ligands, and these longer pathogen-derived peptides maintain a canonical N-terminal binding core yet exhibit a C-terminal extension of 1-30 amino acids. Structural analysis demonstrates that binding of extended peptides opens the HLA class I F' pocket, allowing the C-terminal extension to protrude through one end of the binding groove. In summary, we demonstrate that unrealized structural flexibility makes MHC class I receptive to parasite-derived ligands that exhibit unique C-terminal peptide extensions.

PubMed: 26824387
DOI: 10.7554/eLife.12556

実験手法

X-RAY DIFFRACTION (1.5 Å)