5DAE

Kazal type inhibitor from salivary glands of Aedes aegypti mosquito

Summary for 5DAE

• Entry DOI: 10.2210/pdb5dae/pdb
• Descriptor: AAEL006007-PA (2 entities in total)
• Functional Keywords: inhibitor, kazal, trypsin, anticoagulant, protein binding
• Biological source: Aedes aegypti (Yellowfever mosquito)
• Total number of polymer chains: 2
• Total formula weight: 14724.57

Authors

Torquato, R.J.S.,Pereira, P.J.B.,Tanaka, A.S. (deposition date: 2015-08-19, release date: 2016-09-07, Last modification date: 2024-10-30)

Primary citation

Torquato, R.J.S.,Lu, S.,Martins, N.H.,Tanaka, A.S.,Pereira, P.J.B.
High-resolution structure of a Kazal-type serine protease inhibitor from the dengue vector Aedes aegypti.
Acta Crystallogr F Struct Biol Commun, 73:469-475, 2017

PubMed Abstract: Blood-feeding exoparasites are rich sources of protease inhibitors, and the mosquito Aedes aegypti, which is a vector of Dengue virus, Yellow fever virus, Chikungunya virus and Zika virus, is no exception. AaTI is a single-domain, noncanonical Kazal-type serine proteinase inhibitor from A. aegypti that recognizes both digestive trypsin-like serine proteinases and the central protease in blood clotting, thrombin, albeit with an affinity that is three orders of magnitude lower. Here, the 1.4 Å resolution crystal structure of AaTI is reported from extremely tightly packed crystals (∼22% solvent content), revealing the structural determinants for the observed inhibitory profile of this molecule.

PubMed: 28777090
DOI: 10.1107/S2053230X17010007

Experimental method

X-RAY DIFFRACTION (1.4 Å)