5DAE

Kazal type inhibitor from salivary glands of Aedes aegypti mosquito

5DAE の概要

• エントリーDOI: 10.2210/pdb5dae/pdb
• 分子名称: AAEL006007-PA (2 entities in total)
• 機能のキーワード: inhibitor, kazal, trypsin, anticoagulant, protein binding
• 由来する生物種: Aedes aegypti (Yellowfever mosquito)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 14724.57

構造登録者

Torquato, R.J.S.,Pereira, P.J.B.,Tanaka, A.S. (登録日: 2015-08-19, 公開日: 2016-09-07, 最終更新日: 2024-10-30)

主引用文献

Torquato, R.J.S.,Lu, S.,Martins, N.H.,Tanaka, A.S.,Pereira, P.J.B.
High-resolution structure of a Kazal-type serine protease inhibitor from the dengue vector Aedes aegypti.
Acta Crystallogr F Struct Biol Commun, 73:469-475, 2017

PubMed Abstract: Blood-feeding exoparasites are rich sources of protease inhibitors, and the mosquito Aedes aegypti, which is a vector of Dengue virus, Yellow fever virus, Chikungunya virus and Zika virus, is no exception. AaTI is a single-domain, noncanonical Kazal-type serine proteinase inhibitor from A. aegypti that recognizes both digestive trypsin-like serine proteinases and the central protease in blood clotting, thrombin, albeit with an affinity that is three orders of magnitude lower. Here, the 1.4 Å resolution crystal structure of AaTI is reported from extremely tightly packed crystals (∼22% solvent content), revealing the structural determinants for the observed inhibitory profile of this molecule.

PubMed: 28777090
DOI: 10.1107/S2053230X17010007

実験手法

X-RAY DIFFRACTION (1.4 Å)