5D9V

Crystal structure of oxidized dehydroascorbate reductase (OsDHAR) from Oryza sativa L. japonica

5D9V の概要

• エントリーDOI: 10.2210/pdb5d9v/pdb
• 関連するPDBエントリー: 5d9t 5d9w 5d9x
• 分子名称: Dehydroascorbate reductase, CALCIUM ION, DI(HYDROXYETHYL)ETHER, ... (4 entities in total)
• 機能のキーワード: osdhar, oryza sativa l. japonica, ascorbate, plant protein
• 由来する生物種: Oryza sativa subsp. japonica (Rice)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 25875.52

構造登録者

Do, H.,Lee, J.H. (登録日: 2015-08-19, 公開日: 2016-02-03, 最終更新日: 2025-04-09)

主引用文献

Do, H.,Kim, I.S.,Jeon, B.W.,Lee, C.W.,Park, A.K.,Wi, A.R.,Shin, S.C.,Park, H.,Kim, Y.S.,Yoon, H.S.,Kim, H.W.,Lee, J.H.
Structural understanding of the recycling of oxidized ascorbate by dehydroascorbate reductase (OsDHAR) from Oryza sativa L. japonica
Sci Rep, 6:19498-19498, 2016

PubMed Abstract: Dehydroascorbate reductase (DHAR) is a key enzyme involved in the recycling of ascorbate, which catalyses the glutathione (GSH)-dependent reduction of oxidized ascorbate (dehydroascorbate, DHA). As a result, DHAR regenerates a pool of reduced ascorbate and detoxifies reactive oxygen species (ROS). In previous experiments involving transgenic rice, we observed that overexpression of DHAR enhanced grain yield and biomass. Since the structure of DHAR is not available, the enzymatic mechanism is not well-understood and remains poorly characterized. To elucidate the molecular basis of DHAR catalysis, we determined the crystal structures of DHAR from Oryza sativa L. japonica (OsDHAR) in the native, ascorbate-bound, and GSH-bound forms and refined their resolutions to 1.9, 1.7, and 1.7 Å, respectively. These complex structures provide the first information regarding the location of the ascorbate and GSH binding sites and their interacting residues. The location of the ascorbate-binding site overlaps with the GSH-binding site, suggesting a ping-pong kinetic mechanism for electron transfer at the common Cys20 active site. Our structural information and mutagenesis data provide useful insights into the reaction mechanism of OsDHAR against ROS-induced oxidative stress in rice.

PubMed: 26775680
DOI: 10.1038/srep19498

実験手法

X-RAY DIFFRACTION (1.69 Å)