5D99

3DW4 redetermined by direct methods starting from random phase angles

5D99 の概要

• エントリーDOI: 10.2210/pdb5d99/pdb
• 分子名称: RNA (27-MER) hairpin from sarcin-ricin domain of E. coli 23S rRNA, GLYCEROL (3 entities in total)
• 機能のキーワード: ab initio direct methods structure determination, hairpin rna, rna
• 由来する生物種: Enterobacteria phage L1
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 8850.38

構造登録者

Mooers, B.H.M. (登録日: 2015-08-18, 公開日: 2016-04-13, 最終更新日: 2024-03-06)

主引用文献

Mooers, B.H.
Direct-methods structure determination of a trypanosome RNA-editing substrate fragment with translational pseudosymmetry.
Acta Crystallogr D Struct Biol, 72:477-487, 2016

PubMed Abstract: Using direct methods starting from random phases, the crystal structure of a 32-base-pair RNA (675 non-H RNA atoms in the asymmetric unit) was determined using only the native diffraction data (resolution limit 1.05 Å) and the computer program SIR2014. The almost three helical turns of the RNA in the asymmetric unit introduced partial or imperfect translational pseudosymmetry (TPS) that modulated the intensities when averaged by the l Miller indices but still escaped automated detection. Almost six times as many random phase sets had to be tested on average to reach a correct structure compared with a similar-sized RNA hairpin (27 nucleotides, 580 non-H RNA atoms) without TPS. More sensitive methods are needed for the automated detection of partial TPS.

PubMed: 27050127
DOI: 10.1107/S2059798316001224

実験手法

X-RAY DIFFRACTION (0.97 Å)