5D6C

Structure of 4497 Fab bound to synthetic wall teichoic acid fragment

5D6C の概要

• エントリーDOI: 10.2210/pdb5d6c/pdb
• 分子名称: 4497 antibody IgK (VL and CL), 4497 antibody IgG1 (VH and CH1), CALCIUM ION, ... (7 entities in total)
• 機能のキーワード: staphylococcus aureus wall teichoic acid antibody mrsa, immune system
• 由来する生物種: Homo sapiens; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 97766.68

構造登録者

Lupardus, P.J.,Fong, R. (登録日: 2015-08-12, 公開日: 2015-11-11, 最終更新日: 2024-10-30)

主引用文献

Lehar, S.M.,Pillow, T.,Xu, M.,Staben, L.,Kajihara, K.K.,Vandlen, R.,DePalatis, L.,Raab, H.,Hazenbos, W.L.,Morisaki, J.H.,Kim, J.,Park, S.,Darwish, M.,Lee, B.C.,Hernandez, H.,Loyet, K.M.,Lupardus, P.,Fong, R.,Yan, D.,Chalouni, C.,Luis, E.,Khalfin, Y.,Plise, E.,Cheong, J.,Lyssikatos, J.P.,Strandh, M.,Koefoed, K.,Andersen, P.S.,Flygare, J.A.,Wah Tan, M.,Brown, E.J.,Mariathasan, S.
Novel antibody-antibiotic conjugate eliminates intracellular S. aureus.
Nature, 527:323-328, 2015

PubMed Abstract: Staphylococcus aureus is considered to be an extracellular pathogen. However, survival of S. aureus within host cells may provide a reservoir relatively protected from antibiotics, thus enabling long-term colonization of the host and explaining clinical failures and relapses after antibiotic therapy. Here we confirm that intracellular reservoirs of S. aureus in mice comprise a virulent subset of bacteria that can establish infection even in the presence of vancomycin, and we introduce a novel therapeutic that effectively kills intracellular S. aureus. This antibody-antibiotic conjugate consists of an anti-S. aureus antibody conjugated to a highly efficacious antibiotic that is activated only after it is released in the proteolytic environment of the phagolysosome. The antibody-antibiotic conjugate is superior to vancomycin for treatment of bacteraemia and provides direct evidence that intracellular S. aureus represents an important component of invasive infections.

PubMed: 26536114
DOI: 10.1038/nature16057

実験手法

X-RAY DIFFRACTION (1.72 Å)