5CVY

The Structure of Bacillus pumilus GH48 in complex with cellobiose and cellohexaose

5CVY の概要

• エントリーDOI: 10.2210/pdb5cvy/pdb
• 関連するBIRD辞書のPRD_ID: PRD_900005 PRD_900020
• 分子名称: Glycoside hydrolase, beta-D-glucopyranose-(1-4)-beta-D-glucopyranose, beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose, ... (9 entities in total)
• 機能のキーワード: cellulase, hydrolase
• 由来する生物種: Bacillus pumilus (strain SAFR-032)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 83196.17

構造登録者

Alahuhta, P.M.,Lunin, V.V. (登録日: 2015-07-27, 公開日: 2016-08-10, 最終更新日: 2023-09-27)

主引用文献

Brunecky, R.,Alahuhta, M.,Sammond, D.W.,Xu, Q.,Chen, M.,Wilson, D.B.,Brady, J.W.,Himmel, M.E.,Bomble, Y.J.,Lunin, V.V.
Natural diversity of glycoside hydrolase family 48 exoglucanases: insights from structure.
Biotechnol Biofuels, 10:274-274, 2017

PubMed Abstract: Glycoside hydrolase (GH) family 48 is an understudied and increasingly important exoglucanase family found in the majority of bacterial cellulase systems. Moreover, many thermophilic enzyme systems contain GH48 enzymes. Deletion of GH48 enzymes in these microorganisms results in drastic reduction in biomass deconstruction. Surprisingly, given their importance for these microorganisms, GH48s have intrinsically low cellulolytic activity but even in low ratios synergize greatly with GH9 endoglucanases. In this study, we explore the structural and enzymatic diversity of these enzymes across a wide range of temperature optima. We have crystallized one new GH48 module from in a complex with cellobiose and cellohexaose (GH48). We compare this structure to other known GH48 enzymes in an attempt to understand GH48 structure/function relationships and draw general rules correlating amino acid sequences and secondary structures to thermostability in this GH family.

PubMed: 29213319
DOI: 10.1186/s13068-017-0951-5

実験手法

X-RAY DIFFRACTION (2 Å)