5CTM

Structure of BPu1 beta-lactamase

5CTM の概要

• エントリーDOI: 10.2210/pdb5ctm/pdb
• 関連するPDBエントリー: 5CTN
• 分子名称: Beta-lactamase, CITRATE ANION, 1,2-ETHANEDIOL, ... (5 entities in total)
• 機能のキーワード: hydrolase, beta-lactamase
• 由来する生物種: Bacillus pumilus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 55479.87

構造登録者

Smith, C.A.,Vakulenko, S.B. (登録日: 2015-07-24, 公開日: 2015-11-18, 最終更新日: 2025-04-02)

主引用文献

Toth, M.,Antunes, N.T.,Stewart, N.K.,Frase, H.,Bhattacharya, M.,Smith, C.A.,Vakulenko, S.B.
Class D beta-lactamases do exist in Gram-positive bacteria.
Nat.Chem.Biol., 12:9-14, 2016

PubMed Abstract: Production of β-lactamases of one of four molecular classes (A, B, C and D) is the major mechanism of bacterial resistance to β-lactams, the largest class of antibiotics, which have saved countless lives since their inception 70 years ago. Although several hundred efficient class D enzymes have been identified in Gram-negative pathogens over the last four decades, none have been reported in Gram-positive bacteria. Here we demonstrate that efficient class D β-lactamases capable of hydrolyzing a wide array of β-lactam substrates are widely disseminated in various species of environmental Gram-positive organisms. Class D enzymes of Gram-positive bacteria have a distinct structural architecture and employ a unique substrate-binding mode that is quite different from that of all currently known class A, C and D β-lactamases. These enzymes thus constitute a previously unknown reservoir of novel antibiotic-resistance enzymes.

PubMed: 26551395
DOI: 10.1038/nchembio.1950

実験手法

X-RAY DIFFRACTION (1 Å)