5CHB

Crystal structure of nvPizza2-S16H58 coordinating a CdCl2 nanocrystal

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5CHB の概要

• エントリーDOI: 10.2210/pdb5chb/pdb
• 分子名称: nvPizza2-S16H58, CADMIUM ION, CHLORIDE ION, ... (5 entities in total)
• 機能のキーワード: computational protein design, metalloproteins, cadmium, symmetrical protein, biomineralization, nano-crystal, de novo protein
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 27427.34

構造登録者

Voet, A.R.D.,Noguchi, H.,Addy, C.,Zhang, K.Y.J.,Tame, J.R.H. (登録日: 2015-07-10, 公開日: 2015-07-22, 最終更新日: 2023-11-08)

主引用文献

Voet, A.R.D.,Noguchi, H.,Addy, C.,Zhang, K.Y.J.,Tame, J.R.H.
Biomineralization of a Cadmium Chloride Nanocrystal by a Designed Symmetrical Protein
Angew.Chem.Int.Ed.Engl., 54:9857-9860, 2015

PubMed Abstract: We have engineered a metal-binding site into the novel artificial β-propeller protein Pizza. This new Pizza variant carries two nearly identical domains per polypeptide chain, and forms a trimer with three-fold symmetry. The designed single metal ion binding site lies on the symmetry axis, bonding the trimer together. Two copies of the trimer associate in the presence of cadmium chloride in solution, and very high-resolution X-ray crystallographic analysis reveals a nanocrystal of cadmium chloride, sandwiched between two trimers of the protein. This nanocrystal, containing seven cadmium ions lying in a plane and twelve interspersed chloride ions, is the smallest reported to date. Our results indicate the feasibility of using rationally designed symmetrical proteins to biomineralize nanocrystals with useful properties.

PubMed: 26136355
DOI: 10.1002/anie.201503575

実験手法

X-RAY DIFFRACTION (1.55 Å)