5CCT

Staphylococcus bacteriophage 80alpha dUTPase G164S mutant with dUpNHpp.

Summary for 5CCT

• Entry DOI: 10.2210/pdb5cct/pdb
• Related: 3zez 5CCO
• Descriptor: DUTPase, 2'-DEOXYURIDINE 5'-ALPHA,BETA-IMIDO-TRIPHOSPHATE, MAGNESIUM ION, ... (4 entities in total)
• Functional Keywords: phage, pathogenicity island, sapi induction, gene transfer, moonlighting proteins, dut, dutp, g protein, p-loop, hydrolase
• Biological source: Staphylococcus phage 80alpha
• Total number of polymer chains: 1
• Total formula weight: 19660.46

Authors

Maiques, E.,Quiles-Puchalt, N.,Donderis, J.,Ciges, J.R.,Alite, C.,Bowring, J.,Penades, J.R.,Marina, A. (deposition date: 2015-07-02, release date: 2016-05-11, Last modification date: 2024-01-10)

Primary citation

Maiques, E.,Quiles-Puchalt, N.,Donderis, J.,Ciges-Tomas, J.R.,Alite, C.,Bowring, J.Z.,Humphrey, S.,Penades, J.R.,Marina, A.
Another look at the mechanism involving trimeric dUTPases in Staphylococcus aureus pathogenicity island induction involves novel players in the party.
Nucleic Acids Res., 44:5457-5469, 2016

PubMed Abstract: We have recently proposed that the trimeric staphylococcal phage encoded dUTPases (Duts) are signaling molecules that act analogously to eukaryotic G-proteins, using dUTP as a second messenger. To perform this regulatory role, the Duts require their characteristic extra motif VI, present in all the staphylococcal phage coded trimeric Duts, as well as the strongly conserved Dut motif V. Recently, however, an alternative model involving Duts in the transfer of the staphylococcal islands (SaPIs) has been suggested, questioning the implication of motifs V and VI. Here, using state-of the-art techniques, we have revisited the proposed models. Our results confirm that the mechanism by which the Duts derepress the SaPI cycle depends on dUTP and involves both motifs V and VI, as we have previously proposed. Surprisingly, the conserved Dut motif IV is also implicated in SaPI derepression. However, and in agreement with the proposed alternative model, the dUTP inhibits rather than inducing the process, as we had initially proposed. In summary, our results clarify, validate and establish the mechanism by which the Duts perform regulatory functions.

PubMed: 27112567
DOI: 10.1093/nar/gkw317

Experimental method

X-RAY DIFFRACTION (2.4 Å)