5CCO

Staphylococcus bacteriophage 80alpha dUTPase with dUMP.

5CCO の概要

• エントリーDOI: 10.2210/pdb5cco/pdb
• 関連するPDBエントリー: 3ZEZ
• 分子名称: DUTPase, 2'-DEOXYURIDINE 5'-MONOPHOSPHATE, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: phage, pathogenicity island, sapi induction, gene transfer, moonlighting proteins, dut, dutp, g protein, p-loop, hydrolase
• 由来する生物種: Staphylococcus phage 80alpha
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 19471.46

構造登録者

Maiques, E.,Quiles-Puchalt, N.,Donderis, J.,Ciges, J.R.,Alite, C.,Bowring, J.,Penades, J.R.,Marina, A. (登録日: 2015-07-02, 公開日: 2016-05-11, 最終更新日: 2024-01-10)

主引用文献

Maiques, E.,Quiles-Puchalt, N.,Donderis, J.,Ciges-Tomas, J.R.,Alite, C.,Bowring, J.Z.,Humphrey, S.,Penades, J.R.,Marina, A.
Another look at the mechanism involving trimeric dUTPases in Staphylococcus aureus pathogenicity island induction involves novel players in the party.
Nucleic Acids Res., 44:5457-5469, 2016

PubMed Abstract: We have recently proposed that the trimeric staphylococcal phage encoded dUTPases (Duts) are signaling molecules that act analogously to eukaryotic G-proteins, using dUTP as a second messenger. To perform this regulatory role, the Duts require their characteristic extra motif VI, present in all the staphylococcal phage coded trimeric Duts, as well as the strongly conserved Dut motif V. Recently, however, an alternative model involving Duts in the transfer of the staphylococcal islands (SaPIs) has been suggested, questioning the implication of motifs V and VI. Here, using state-of the-art techniques, we have revisited the proposed models. Our results confirm that the mechanism by which the Duts derepress the SaPI cycle depends on dUTP and involves both motifs V and VI, as we have previously proposed. Surprisingly, the conserved Dut motif IV is also implicated in SaPI derepression. However, and in agreement with the proposed alternative model, the dUTP inhibits rather than inducing the process, as we had initially proposed. In summary, our results clarify, validate and establish the mechanism by which the Duts perform regulatory functions.

PubMed: 27112567
DOI: 10.1093/nar/gkw317

実験手法

X-RAY DIFFRACTION (2.33 Å)