5CCK

Crystal structure of Human anti-HIV-1 broadly neutralizing antibody 3BC315 Fab

5CCK の概要

• エントリーDOI: 10.2210/pdb5cck/pdb
• 関連するPDBエントリー: 5AWN
• EMDBエントリー: 3067
• 分子名称: Antibody 3BC315 Fab heavy chain, Antibody 3BC315 Fab light chain, 3-CYCLOHEXYL-1-PROPYLSULFONIC ACID, ... (4 entities in total)
• 機能のキーワード: hiv-1, env, bnab, antibody, immune system
• 由来する生物種: Homo sapiens; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 48230.16

構造登録者

Lee, J.H.,Ward, A.B.,Wilson, I.A. (登録日: 2015-07-02, 公開日: 2015-10-07, 最終更新日: 2024-10-16)

主引用文献

Lee, J.H.,Leaman, D.P.,Kim, A.S.,Torrents de la Pena, A.,Sliepen, K.,Yasmeen, A.,Derking, R.,Ramos, A.,de Taeye, S.W.,Ozorowski, G.,Klein, F.,Burton, D.R.,Nussenzweig, M.C.,Poignard, P.,Moore, J.P.,Klasse, P.J.,Sanders, R.W.,Zwick, M.B.,Wilson, I.A.,Ward, A.B.
Antibodies to a conformational epitope on gp41 neutralize HIV-1 by destabilizing the Env spike.
Nat Commun, 6:8167-8167, 2015

PubMed Abstract: The recent identification of three broadly neutralizing antibodies (bnAbs) against gp120-gp41 interface epitopes has expanded the targetable surface on the HIV-1 envelope glycoprotein (Env) trimer. By using biochemical, biophysical and computational methods, we map the previously unknown trimer epitopes of two related antibodies, 3BC315 and 3BC176. A cryo-EM reconstruction of a soluble Env trimer bound to 3BC315 Fab at 9.3 Å resolution reveals that the antibody binds between two gp41 protomers, and neutralizes the virus by accelerating trimer decay. In contrast, bnAb 35O22 binding to a partially overlapping quaternary epitope at the gp120-gp41 interface does not induce decay. A conserved gp41-proximal glycan at N88 was also shown to play a role in the binding kinetics of 3BC176 and 3BC315. Finally, our data suggest that the dynamic structure of the Env trimer influences exposure of bnAb epitopes.

PubMed: 26404402
DOI: 10.1038/ncomms9167

実験手法

X-RAY DIFFRACTION (1.95 Å)