5C9H

Structural Basis of Template Boundary Definition in Tetrahymena Telomerase

5C9H の概要

• エントリーDOI: 10.2210/pdb5c9h/pdb
• 分子名称: Telomerase reverse transcriptase, RNA (5'-R(P*AP*GP*AP*AP*CP*UP*GP*UP*CP*A)-3'), RNA (5'-R(P*UP*CP*AP*UP*UP*CP*AP*GP*UP*UP*CP*U)-3'), ... (5 entities in total)
• 機能のキーワード: telomerase, rna-protein complex, rna binding protein-rna complex, rna binding protein/rna
• 由来する生物種: Tetrahymena thermophila; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 89821.44

構造登録者

Jansson, L.I.,Akiyama, B.M.,Ooms, A.,Lu, C.,Rubin, S.M.,Stone, M.D. (登録日: 2015-06-26, 公開日: 2015-10-14, 最終更新日: 2023-09-27)

主引用文献

Jansson, L.I.,Akiyama, B.M.,Ooms, A.,Lu, C.,Rubin, S.M.,Stone, M.D.
Structural basis of template-boundary definition in Tetrahymena telomerase.
Nat.Struct.Mol.Biol., 22:883-888, 2015

PubMed Abstract: Telomerase is required to maintain repetitive G-rich telomeric DNA sequences at chromosome ends. To do so, the telomerase reverse transcriptase (TERT) subunit reiteratively uses a small region of the integral telomerase RNA (TER) as a template. An essential feature of telomerase catalysis is the strict definition of the template boundary to determine the precise TER nucleotides to be reverse transcribed by TERT. We report the 3-Å crystal structure of the Tetrahymena TERT RNA-binding domain (tTRBD) bound to the template boundary element (TBE) of TER. tTRBD is wedged into the base of the TBE RNA stem-loop, and each of the flanking RNA strands wraps around opposite sides of the protein domain. The structure illustrates how the tTRBD establishes the template boundary by positioning the TBE at the correct distance from the TERT active site to prohibit copying of nontemplate nucleotides.

PubMed: 26436828
DOI: 10.1038/nsmb.3101

実験手法

X-RAY DIFFRACTION (3 Å)