5C98

1.45A resolution structure of SRPN18 from Anopheles gambiae

5C98 の概要

• エントリーDOI: 10.2210/pdb5c98/pdb
• 分子名称: AGAP007691-PB (2 entities in total)
• 機能のキーワード: serpin, serine protease, insect immunity, enzyme inhibitor, unknown function
• 由来する生物種: Anopheles gambiae (African malaria mosquito)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 86357.48

構造登録者

Lovell, S.,Battaile, K.P.,Gulley, M.,Zhang, X.,Meekins, D.A.,Gao, F.P.,Michel, K. (登録日: 2015-06-26, 公開日: 2016-09-14, 最終更新日: 2023-09-27)

主引用文献

Meekins, D.A.,Zhang, X.,Battaile, K.P.,Lovell, S.,Michel, K.
1.45 angstrom resolution structure of SRPN18 from the malaria vector Anopheles gambiae.
Acta Crystallogr F Struct Biol Commun, 72:853-862, 2016

PubMed Abstract: Serine protease inhibitors (serpins) in insects function within development, wound healing and immunity. The genome of the African malaria vector, Anopheles gambiae, encodes 23 distinct serpin proteins, several of which are implicated in disease-relevant physiological responses. A. gambiae serpin 18 (SRPN18) was previously categorized as non-inhibitory based on the sequence of its reactive-center loop (RCL), a region responsible for targeting and initiating protease inhibition. The crystal structure of A. gambiae SRPN18 was determined to a resolution of 1.45 Å, including nearly the entire RCL in one of the two molecules in the asymmetric unit. The structure reveals that the SRPN18 RCL is extremely short and constricted, a feature associated with noncanonical inhibitors or non-inhibitory serpin superfamily members. Furthermore, the SRPN18 RCL does not contain a suitable protease target site and contains a large number of prolines. The SRPN18 structure therefore reveals a unique RCL architecture among the highly conserved serpin fold.

PubMed: 27917832
DOI: 10.1107/S2053230X16017854

実験手法

X-RAY DIFFRACTION (1.45 Å)