5C50

Crystal structure of the complex of human Atg101-Atg13 HORMA domain

5C50 の概要

• エントリーDOI: 10.2210/pdb5c50/pdb
• 分子名称: Autophagy-related protein 101, Autophagy-related protein 13, BENZAMIDINE, ... (4 entities in total)
• 機能のキーワード: complex, protein binding, autophagy, horma domain
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 45056.89

構造登録者

Qi, S.,Hurley, J.H. (登録日: 2015-06-19, 公開日: 2015-10-14, 最終更新日: 2024-03-06)

主引用文献

Qi, S.,Kim, D.J.,Stjepanovic, G.,Hurley, J.H.
Structure of the Human Atg13-Atg101 HORMA Heterodimer: an Interaction Hub within the ULK1 Complex.
Structure, 23:1848-1857, 2015

PubMed Abstract: The ULK1 complex, consisting of the ULK1 protein kinase itself, FIP200, Atg13, and Atg101, controls the initiation of autophagy in animals. We determined the structure of the complex of the human Atg13 HORMA (Hop1, Rev7, Mad2) domain in complex with the full-length HORMA domain-only protein Atg101. The two HORMA domains assemble with an architecture conserved in the Mad2 conformational heterodimer and the S. pombe Atg13-Atg101 HORMA complex. The WF finger motif that is essential for function in human Atg101 is sequestered in a hydrophobic pocket, suggesting that the exposure of this motif is regulated. Benzamidine molecules from the crystallization solution mark two hydrophobic pockets that are conserved in, and unique to, animals, and are suggestive of sites that could interact with other proteins. These features suggest that the activity of the animal Atg13-Atg101 subcomplex is regulated and that it is an interaction hub for multiple partners.

PubMed: 26299944
DOI: 10.1016/j.str.2015.07.011

実験手法

X-RAY DIFFRACTION (1.63 Å)