5BSX
Crystal structure of GII.10 P domain in complex with disinfectant Puregreen24
Summary for 5BSX
| Entry DOI | 10.2210/pdb5bsx/pdb |
| Descriptor | Capsid protein, CITRATE ANION, 1,2-ETHANEDIOL, ... (4 entities in total) |
| Functional Keywords | citrate, norovirus, protruding domain, viral protein |
| Biological source | Norwalk virus |
| Total number of polymer chains | 2 |
| Total formula weight | 69388.62 |
| Authors | Koromyslova, A.D.,Hansman, G.S. (deposition date: 2015-06-02, release date: 2015-09-09, Last modification date: 2024-01-10) |
| Primary citation | Koromyslova, A.D.,White, P.A.,Hansman, G.S. Treatment of norovirus particles with citrate. Virology, 485:199-204, 2015 Cited by PubMed Abstract: Human norovirus is a dominant cause of acute gastroenteritis around the world. Several norovirus disinfectants label citric acid as an active ingredient. In this study, we showed that norovirus virus-like particles (VLPs) treated with citrate buffer caused the particles to alter their morphology, including increased diameters associated with a new ring-like structure. We also found that epitopes on the protruding (P) domain on these particles were more readily accessible to antibodies after the citrate treatment. These results suggested that citrate had a direct effect on the norovirus particles. Using X-ray crystallography, we showed that the P domain bound citrate from lemon juice and a disinfectant containing citric acid. Importantly, citrate binds at the histo-blood group antigen binding pocket, which are attachment factors for norovirus infections. Taken together, these new findings suggested that it might be possible to treat/reduce norovirus infections with citrate, although further studies are needed. PubMed: 26295280DOI: 10.1016/j.virol.2015.07.009 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.78 Å) |
Structure validation
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