5BSX

Crystal structure of GII.10 P domain in complex with disinfectant Puregreen24

5BSX の概要

• エントリーDOI: 10.2210/pdb5bsx/pdb
• 分子名称: Capsid protein, CITRATE ANION, 1,2-ETHANEDIOL, ... (4 entities in total)
• 機能のキーワード: citrate, norovirus, protruding domain, viral protein
• 由来する生物種: Norwalk virus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 69388.62

構造登録者

Koromyslova, A.D.,Hansman, G.S. (登録日: 2015-06-02, 公開日: 2015-09-09, 最終更新日: 2024-01-10)

主引用文献

Koromyslova, A.D.,White, P.A.,Hansman, G.S.
Treatment of norovirus particles with citrate.
Virology, 485:199-204, 2015

PubMed Abstract: Human norovirus is a dominant cause of acute gastroenteritis around the world. Several norovirus disinfectants label citric acid as an active ingredient. In this study, we showed that norovirus virus-like particles (VLPs) treated with citrate buffer caused the particles to alter their morphology, including increased diameters associated with a new ring-like structure. We also found that epitopes on the protruding (P) domain on these particles were more readily accessible to antibodies after the citrate treatment. These results suggested that citrate had a direct effect on the norovirus particles. Using X-ray crystallography, we showed that the P domain bound citrate from lemon juice and a disinfectant containing citric acid. Importantly, citrate binds at the histo-blood group antigen binding pocket, which are attachment factors for norovirus infections. Taken together, these new findings suggested that it might be possible to treat/reduce norovirus infections with citrate, although further studies are needed.

PubMed: 26295280
DOI: 10.1016/j.virol.2015.07.009

実験手法

X-RAY DIFFRACTION (1.78 Å)