5BQ7

Crystal structure of chikungunya virus-human Fab 5F-10 fragment

5BQ7 の概要

• エントリーDOI: 10.2210/pdb5bq7/pdb
• 分子名称: Fab 5F-10-Heavy Chain, Fab 5F-10-Light Chain, DI(HYDROXYETHYL)ETHER, ... (4 entities in total)
• 機能のキーワード: human antibody fab fragment, immune system
• 由来する生物種: Homo sapiens; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 50572.56

構造登録者

Mangala Prasad, V.,Rossmann, M.G. (登録日: 2015-05-28, 公開日: 2015-11-18, 最終更新日: 2023-09-27)

主引用文献

Porta, J.,Mangala Prasad, V.,Wang, C.I.,Akahata, W.,Ng, L.F.,Rossmann, M.G.
Structural Studies of Chikungunya Virus-Like Particles Complexed with Human Antibodies: Neutralization and Cell-to-Cell Transmission.
J.Virol., 90:1169-1177, 2015

PubMed Abstract: Chikungunya virus is a positive-stranded RNA alphavirus. Structures of chikungunya virus-like particles in complex with strongly neutralizing antibody Fab fragments (8B10 and 5F10) were determined using cryo-electron microscopy and X-ray crystallography. By fitting the crystallographically determined structures of these Fab fragments into the cryo-electron density maps, we show that Fab fragments of antibody 8B10 extend radially from the viral surface and block receptor binding on the E2 glycoprotein. In contrast, Fab fragments of antibody 5F10 bind the tip of the E2 B domain and lie tangentially on the viral surface. Fab 5F10 fixes the B domain rigidly to the surface of the virus, blocking exposure of the fusion loop on glycoprotein E1 and therefore preventing the virus from becoming fusogenic. Although Fab 5F10 can neutralize the wild-type virus, it can also bind to a mutant virus without inhibiting fusion or attachment. Although the mutant virus is no longer able to propagate by extracellular budding, it can, however, enter the next cell by traveling through junctional complexes without being intercepted by a neutralizing antibody to the wild-type virus, thus clarifying how cell-to-cell transmission can occur.

PubMed: 26537684
DOI: 10.1128/JVI.02364-15

実験手法

X-RAY DIFFRACTION (2.738 Å)