5BPX

Structure of the 2,4'-dihydroxyacetophenone dioxygenase from Alcaligenes sp. 4HAP.

5BPX の概要

• エントリーDOI: 10.2210/pdb5bpx/pdb
• 関連するPDBエントリー: 4p9g
• 分子名称: 2,4'-dihydroxyacetophenone dioxygenase, FE (III) ION, ACETATE ION, ... (5 entities in total)
• 機能のキーワード: rmlc-like cupin protein, oxidoreductase
• 由来する生物種: Alcaligenes sp.
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 17950.83

構造登録者

Guo, J.,Erskine, P.,Wood, S.P.,Cooper, J.B. (登録日: 2015-05-28, 公開日: 2015-06-10, 最終更新日: 2024-01-10)

主引用文献

Guo, J.,Erskine, P.,Coker, A.R.,Gor, J.,Perkins, S.J.,Wood, S.P.,Cooper, J.B.
Extension of resolution and oligomerization-state studies of 2,4'-dihydroxyacetophenone dioxygenase from Alcaligenes sp. 4HAP.
Acta Crystallogr.,Sect.F, 71:1258-1263, 2015

PubMed Abstract: The enzyme 2,4'-dihydroxyacetophenone dioxygenase (DAD) catalyses the conversion of 2,4'-dihydroxyacetophenone to 4-hydroxybenzoic acid and formic acid. This enzyme is a very unusual dioxygenase in that it cleaves a C-C bond in a substituent of the aromatic ring rather than within the ring itself. Whilst it has been shown that DAD is a tetramer in solution, the recently solved crystal structure of the Alcaligenes sp. 4HAP enzyme was in fact dimeric rather than tetrameric. Since the use of limited chymotrypsinolysis, which apparently results in removal of the first 20 or so N-terminal residues of DAD, was necessary for crystallization of the protein, it was investigated whether this was responsible for the change in its oligomerization state. Gel-filtration and analytical ultracentrifugation studies were conducted, which confirmed that chymotrypsinolysed DAD has an apparent molecular weight of around 40 kDa, corresponding to a dimer. In contrast, the native enzyme has a molecular weight in the 70-80 kDa region, as expected for the tetramer. The structural basis for tetramerization has been investigated by the use of several docking servers, and the results are remarkably consistent with the tetrameric structure of a homologous cupin protein from Ralstonia eutropha (PDB entry 3ebr).

PubMed: 26457516
DOI: 10.1107/S2053230X15015873

実験手法

X-RAY DIFFRACTION (1.88 Å)