5BOI

Bacillus megaterium YpeB C-terminal domain

Summary for 5BOI

• Entry DOI: 10.2210/pdb5boi/pdb
• Descriptor: Germination protein YpeB, SULFATE ION (3 entities in total)
• Functional Keywords: pepsy domain, inhibitory protein, unknown function
• Biological source: Bacillus megaterium QM B1551
• Total number of polymer chains: 1
• Total formula weight: 28398.02

Authors

Christie, G.,Chirgadze, D.Y.,Ustok, F.I. (deposition date: 2015-05-27, release date: 2015-08-12, Last modification date: 2024-05-08)

Primary citation

Ustok, F.I.,Chirgadze, D.Y.,Christie, G.
Crystal structure of the PepSY-containing domain of the YpeB protein involved in germination of bacillus spores.
Proteins, 83:1914-1921, 2015

PubMed Abstract: The crystal structure of the C-terminal domain of the Bacillus megaterium YpeB protein has been solved by X-ray crystallography to 1.80-Å resolution. The full-length protein is essential in stabilising the SleB cortex lytic enzyme in Bacillus spores, and may have a role in regulating SleB activity during spore germination. The YpeB-C crystal structure comprises three tandemly repeated PepSY domains, which are aligned to form an extended laterally compressed molecule. A predominantly positively charged region located in the second PepSY domain may provide a site for protein interactions that are important in stabilising SleB and YpeB within the spore.

PubMed: 26219275
DOI: 10.1002/prot.24868

Experimental method

X-RAY DIFFRACTION (1.8 Å)