5BOB

Crystal Structure of the Meningitis Pathogen Streptococcus suis adhesion Fhb

5BOB の概要

• エントリーDOI: 10.2210/pdb5bob/pdb
• 関連するPDBエントリー: 5BOA
• 分子名称: Translation initiation factor 2 (IF-2 GTPase), GLYCEROL (3 entities in total)
• 機能のキーワード: beta sandwich core, translation
• 由来する生物種: Streptococcus suis (strain 05ZYH33)
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 129637.39

構造登録者

Jiang, Y.,Zhang, C.,Yu, Y. (登録日: 2015-05-27, 公開日: 2015-11-18, 最終更新日: 2024-11-13)

主引用文献

Zhang, C.,Yu, Y.,Yang, M.,Jiang, Y.
Expression, purification, crystallization and structure determination of the N terminal domain of Fhb, a factor H binding protein from Streptococcus suis.
Biochem.Biophys.Res.Commun., 466:413-417, 2015

PubMed Abstract: Fhb is a surface virulence protein from Streptococcus suis, which could aid bacterial evasion of host innate immune defense by recruiting complement regulator factor H to inactivate C3b deposited on bacterial surface in blood. Here we successfully expressed and purified the N terminal domain of Fhb (N-Fhb) and obtained crystals of the N-Fhb by sitting-drop vapor diffusion method with a resolution of 1.50 Å. The crystals belong to space group C2 with unit cell parameters a = 127.1 Å, b = 77.3 Å, c = 131.6 Å, α = 90°, β = 115.9°, γ = 90°. The structure of N-Fhb was determined by SAD method and the core structure of N-Fhb is a β sandwich. We speculated that binding of Fhb to human factor H may be mainly mediated by surface amino acids with negative charges.

PubMed: 26365348
DOI: 10.1016/j.bbrc.2015.09.040

実験手法

X-RAY DIFFRACTION (1.5 Å)