5BNA

THE PRIMARY MODE OF BINDING OF CISPLATIN TO A B-DNA DODECAMER: C-G-C-G-A-A-T-T-C-G-C-G

5BNA の概要

• エントリーDOI: 10.2210/pdb5bna/pdb
• 分子名称: DNA (5'-D(*CP*GP*CP*GP*AP*AP*TP*TP*CP*GP*CP*G)-3'), PLATINUM TRIAMINE ION (3 entities in total)
• 機能のキーワード: b-dna, double helix, complexed with drug, modified, dna
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 8065.29

構造登録者

Wing, R.M.,Pjura, P.,Drew, H.R.,Dickerson, R.E. (登録日: 1983-08-22, 公開日: 1983-11-02, 最終更新日: 2024-03-06)

主引用文献

Wing, R.M.,Pjura, P.,Drew, H.R.,Dickerson, R.E.
The primary mode of binding of cisplatin to a B-DNA dodecamer: C-G-C-G-A-A-T-T-C-G-C-G
EMBO J., 3:1201-1206, 1984

PubMed Abstract: When cisplatin [cis- diamminodichloroplatinum (II)] is diffused into pre-grown crystals of the B-DNA double-helical dodecamer C-G-C-G-A-A-T-T-C-G-C-G, it binds preferentially to the N7 positions of guanines, with what probably is an aquo bridge between Pt and the adjacent O6 atom of the same guanine. The entire guanine ring moves slightly toward the platinum site, into the major groove. Only three of the eight potential cisplatin binding sites on guanines actually are occupied, and this differential reactivity can be explained in terms of the relative freedom of motion of guanines toward the major groove. This shift of guanines upon ligation may weaken the glycosyl bond and assist in the depurination that leads to mismatch SOS repair and G.C. to T.A. transversion.

PubMed: 6539674

実験手法

X-RAY DIFFRACTION (2.6 Å)