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5B60

Crystal structure of PtLCIB4 S47R mutant, a homolog of the limiting CO2-inducible protein LCIB

5B60 の概要
エントリーDOI10.2210/pdb5b60/pdb
関連するPDBエントリー5B5X 5B5Y 5B5Z 5K5W
分子名称PtLCIB4 S47R mutant, ZINC ION, CHLORIDE ION, ... (4 entities in total)
機能のキーワードmetalloenzyme, metal binding protein
由来する生物種Phaeodactylum tricornutum
タンパク質・核酸の鎖数1
化学式量合計30478.85
構造登録者
Jin, S.,Sun, J.,Wunder, T.,Tang, D.,Mueller-Caja, O.M.,Gao, Y. (登録日: 2016-05-24, 公開日: 2016-12-07, 最終更新日: 2023-11-08)
主引用文献Jin, S.,Sun, J.,Wunder, T.,Tang, D.,Cousins, A.B.,Sze, S.K.,Mueller-Cajar, O.,Gao, Y.G.
Structural insights into the LCIB protein family reveals a new group of beta-carbonic anhydrases
Proc. Natl. Acad. Sci. U.S.A., 113:14716-14721, 2016
Cited by
PubMed Abstract: Aquatic microalgae have evolved diverse CO-concentrating mechanisms (CCMs) to saturate the carboxylase with its substrate, to compensate for the slow kinetics and competing oxygenation reaction of the key photosynthetic CO-fixing enzyme rubisco. The limiting CO-inducible B protein (LCIB) is known to be essential for CCM function in Chlamydomonas reinhardtii To assign a function to this previously uncharacterized protein family, we purified and characterized a phylogenetically diverse set of LCIB homologs. Three of the six homologs are functional carbonic anhydrases (CAs). We determined the crystal structures of LCIB and limiting CO-inducible C protein (LCIC) from C. reinhardtii and a CA-functional homolog from Phaeodactylum tricornutum, all of which harbor motifs bearing close resemblance to the active site of canonical β-CAs. Our results identify the LCIB family as a previously unidentified group of β-CAs, and provide a biochemical foundation for their function in the microalgal CCMs.
PubMed: 27911826
DOI: 10.1073/pnas.1616294113
主引用文献が同じPDBエントリー
実験手法
X-RAY DIFFRACTION (2.2 Å)
構造検証レポート
Validation report summary of 5b60
検証レポート(詳細版)ダウンロードをダウンロード

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件を2025-01-29に公開中

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