5B5N の概要
| エントリーDOI | 10.2210/pdb5b5n/pdb |
| 関連するPDBエントリー | 5B5M |
| 分子名称 | Photosynthetic reaction center cytochrome c subunit, BACTERIOPHEOPHYTIN A, Ubiquinone-8, ... (18 entities in total) |
| 機能のキーワード | bacterial photosynthesis, light-harvesting complex, photosynthesis |
| 由来する生物種 | Thermochromatium tepidum 詳細 |
| タンパク質・核酸の鎖数 | 72 |
| 化学式量合計 | 782466.01 |
| 構造登録者 | |
| 主引用文献 | Yu, L.J.,Kawakami, T.,Kimura, Y.,Wang-Otomo, Z.Y. Structural Basis for the Unusual Qy Red-Shift and Enhanced Thermostability of the LH1 Complex from Thermochromatium tepidum. Biochemistry, 55:6495-6504, 2016 Cited by PubMed Abstract: While the majority of the core light-harvesting complexes (LH1) in purple photosynthetic bacteria exhibit a Q absorption band in the range of 870-890 nm, LH1 from the thermophilic bacterium Thermochromatium tepidum displays the Q band at 915 nm with an enhanced thermostability. These properties are regulated by Ca ions. Substitution of the Ca with other divalent metal ions results in a complex with the Q band blue-shifted to 880-890 nm and a reduced thermostability. Following the recent publication of the structure of the Ca-bound LH1-reaction center (RC) complex [Niwa, S., et al. (2014) Nature 508, 228], we have determined the crystal structures of the Sr- and Ba-substituted LH1-RC complexes with the LH1 Q band at 888 nm. Sixteen Sr and Ba ions are identified in the LH1 complexes. Both Sr and Ba are located at the same positions, and these are clearly different from, though close to, the Ca-binding sites. Conformational rearrangement induced by the substitution is limited to the metal-binding sites. Unlike the Ca-LH1-RC complex, only the α-polypeptides are involved in the Sr and Ba coordinations in LH1. The difference in the thermostability between these complexes can be attributed to the different patterns of the network formed by metal binding. The Sr- and Ba-LH1-RC complexes form a single-ring network by the LH1 α-polypeptides only, in contrast to the double-ring network composed of both α- and β-polypeptides in the Ca-LH1-RC complex. On the basis of the structural information, a combined effect of hydrogen bonding, structural integrity, and charge distribution is considered to influence the spectral properties of the core antenna complex. PubMed: 27933779DOI: 10.1021/acs.biochem.6b00742 主引用文献が同じPDBエントリー |
| 実験手法 | X-RAY DIFFRACTION (3.3 Å) |
構造検証レポート
検証レポート(詳細版)
をダウンロード
をダウンロード
