5B1L

The mouse nucleosome structure containing H3t

Summary for 5B1L

• Entry DOI: 10.2210/pdb5b1l/pdb
• Related: 5B1M
• Descriptor: Histone H3t, Histone H4, Histone H2A type 1, ... (8 entities in total)
• Functional Keywords: chromatin, spermatogenesis, histone-fold, structural protein-dna complex, structural protein/dna
• Biological source: Mus musculus (Mouse); More
• Total number of polymer chains: 10
• Total formula weight: 203316.79

Authors

Urahama, T.,Machida, S.,Horikoshi, N.,Osakabe, A.,Tachiwana, H.,Taguchi, H.,Kurumizaka, H. (deposition date: 2015-12-08, release date: 2017-02-15, Last modification date: 2023-11-08)

Primary citation

Ueda, J.,Harada, A.,Urahama, T.,Machida, S.,Maehara, K.,Hada, M.,Makino, Y.,Nogami, J.,Horikoshi, N.,Osakabe, A.,Taguchi, H.,Tanaka, H.,Tachiwana, H.,Yao, T.,Yamada, M.,Iwamoto, T.,Isotani, A.,Ikawa, M.,Tachibana, T.,Okada, Y.,Kimura, H.,Ohkawa, Y.,Kurumizaka, H.,Yamagata, K.
Testis-Specific Histone Variant H3t Gene Is Essential for Entry into Spermatogenesis
Cell Rep, 18:593-600, 2017

PubMed Abstract: Cellular differentiation is associated with dynamic chromatin remodeling in establishing a cell-type-specific epigenomic landscape. Here, we find that mouse testis-specific and replication-dependent histone H3 variant H3t is essential for very early stages of spermatogenesis. H3t gene deficiency leads to azoospermia because of the loss of haploid germ cells. When differentiating spermatogonia emerge in normal spermatogenesis, H3t appears and replaces the canonical H3 proteins. Structural and biochemical analyses reveal that H3t-containing nucleosomes are more flexible than the canonical nucleosomes. Thus, by incorporating H3t into the genome during spermatogonial differentiation, male germ cells are able to enter meiosis and beyond.

PubMed: 28099840
DOI: 10.1016/j.celrep.2016.12.065

Experimental method

X-RAY DIFFRACTION (2.35 Å)