5B0U

Crystal structure of the mutated 19 kDa protein of Oplophorus luciferase (nanoKAZ)

Summary for 5B0U

• Entry DOI: 10.2210/pdb5b0u/pdb
• Descriptor: Oplophorus-luciferin 2-monooxygenase catalytic subunit (2 entities in total)
• Functional Keywords: oplophorus luciferase, luminescent protein
• Biological source: Oplophorus gracilirostris (Luminous shrimp)
• Total number of polymer chains: 2
• Total formula weight: 43035.04

Authors

Tomabechi, Y.,Ehara, H.,Sekine, S.I.,Shirouzu, M. (deposition date: 2015-11-04, release date: 2016-01-13, Last modification date: 2024-03-20)

Primary citation

Tomabechi, Y.,Hosoya, T.,Ehara, H.,Sekine, S.,Shirouzu, M.,Inouye, S.
Crystal structure of nanoKAZ: The mutated 19 kDa component of Oplophorus luciferase catalyzing the bioluminescent reaction with coelenterazine
Biochem.Biophys.Res.Commun., 470:88-93, 2016

PubMed Abstract: The 19 kDa protein (KAZ) of Oplophorus luciferase is a catalytic component, that oxidizes coelenterazine (a luciferin) with molecular oxygen to emit light. The crystal structure of the mutated 19 kDa protein (nanoKAZ) was determined at 1.71 Å resolution. The structure consists of 11 antiparallel β-strands forming a β-barrel that is capped by 4 short α-helices. The structure of nanoKAZ is similar to those of fatty acid-binding proteins (FABPs), even though the amino acid sequence similarity was very low between them. The coelenterazine-binding site and the catalytic site for the luminescence reaction might be in a central cavity of the β-barrel structure.

PubMed: 26746005
DOI: 10.1016/j.bbrc.2015.12.123

Experimental method

X-RAY DIFFRACTION (1.71 Å)