5B0R

Beta-1,2-Mannobiose phosphorylase from Listeria innocua - beta-1,2-mannobiose complex

5B0R の概要

• エントリーDOI: 10.2210/pdb5b0r/pdb
• 関連するPDBエントリー: 5B0P 5B0Q 5B0S
• 分子名称: Lin0857 protein, beta-D-mannopyranose-(1-2)-beta-D-mannopyranose, SULFATE ION, ... (6 entities in total)
• 機能のキーワード: glycoside phosphorylase, transferase
• 由来する生物種: Listeria innocua Clip11262
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 84432.23

構造登録者

Tsuda, T.,Arakawa, T.,Fushinobu, S. (登録日: 2015-11-02, 公開日: 2015-12-02, 最終更新日: 2023-11-08)

主引用文献

Tsuda, T.,Nihira, T.,Chiku, K.,Suzuki, E.,Arakawa, T.,Nishimoto, M.,Kitaoka, M.,Nakai, H.,Fushinobu, S.
Characterization and crystal structure determination of beta-1,2-mannobiose phosphorylase from Listeria innocua
Febs Lett., 589:3816-3821, 2015

PubMed Abstract: Glycoside hydrolase family 130 consists of phosphorylases and hydrolases for β-mannosides. Here, we characterized β-1,2-mannobiose phosphorylase from Listeria innocua (Lin0857) and determined its crystal structures complexed with β-1,2-linked mannooligosaccharides. β-1,2-Mannotriose was bound in a U-shape, interacting with a phosphate analog at both ends. Lin0857 has a unique dimer structure connected by a loop, and a significant open-close loop displacement was observed for substrate entry. A long loop, which is exclusively present in Lin0857, covers the active site to limit the pocket size. A structural basis for substrate recognition and phosphorolysis was provided.

PubMed: 26632508
DOI: 10.1016/j.febslet.2015.11.034

実験手法

X-RAY DIFFRACTION (1.8 Å)