5AXF

Crystal Structure Analysis of DNA Duplexes containing sulfoamide-bridged nucleic acid (SuNA-NMe)

5AXF の概要

• エントリーDOI: 10.2210/pdb5axf/pdb
• 関連するPDBエントリー: 5AXE
• 分子名称: DNA (5'-D(*GP*CP*GP*TP*AP*(LSM)P*AP*CP*GP*C)-3') (2 entities in total)
• 機能のキーワード: a-form dna, sulfonamide-bridged nucleic acid, suna[nme], antisense, dna
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 6300.24

構造登録者

Mitsuoka, Y.,Aoyama, H.,Kugimiya, A.,Fujimura, Y.,Yamamoto, T.,Waki, R.,Wada, F.,Tahara, S.,Sawamura, M.,Noda, M.,Hari, Y.,Obika, S. (登録日: 2015-07-28, 公開日: 2016-07-20, 最終更新日: 2024-03-20)

主引用文献

Mitsuoka, Y.,Aoyama, H.,Kugimiya, A.,Fujimura, Y.,Yamamoto, T.,Waki, R.,Wada, F.,Tahara, S.,Sawamura, M.,Noda, M.,Hari, Y.,Obika, S.
Effect of an N-substituent in sulfonamide-bridged nucleic acid (SuNA) on hybridization ability and duplex structure.
Org.Biomol.Chem., 14:6531-6538, 2016

PubMed Abstract: A sulfonamide-bridged nucleic acid without an N-substituent (SuNA[NH]) was successfully synthesized. A comparison of the SuNA[NMe]- and SuNA[NH]-modified oligonucleotides revealed that the duplex-forming abilities of the SuNA[NMe]-modified oligonucleotides with complementary DNA and RNA were higher than those of the SuNA[NH]-modified oligonucleotides. The crystal structures of DNA duplexes containing a SuNA[NR] revealed that the helical structures of the two duplexes and hydration patterns around the bridge moiety were different. These results provide insights into hydration patterns and rationale for the high RNA affinity of SuNA-modified oligonucleotides.

PubMed: 27296230
DOI: 10.1039/c6ob01051b

実験手法

X-RAY DIFFRACTION (1.13 Å)