5AO8

Crystal Structure of SltB3 from Pseudomonas aeruginosa in complex with NAG-NAM-pentapeptide

5AO8 の概要

• エントリーDOI: 10.2210/pdb5ao8/pdb
• 関連するPDBエントリー: 5ANZ 5AO7
• 分子名称: SOLUBLE LYTIC TRANGLYCOSILASE B3, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-methyl 2-acetamido-3-O-[(2R)-1-amino-1-oxopropan-2-yl]-2-deoxy-beta-D-glucopyranoside, CALCIUM ION, ... (4 entities in total)
• 機能のキーワード: transferase, cell wall recycling
• 由来する生物種: PSEUDOMONAS AERUGINOSA
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 45805.22

構造登録者

Dominguez-Gil, T.,Hermoso, J.A. (登録日: 2015-09-09, 公開日: 2016-07-20, 最終更新日: 2024-01-10)

主引用文献

Lee, M.,Dominguez-Gil, T.,Hesek, D.,Mahasenan, K.V.,Lastochkin, E.,Hermoso, J.A.,Mobashery, S.
Turnover of Bacterial Cell Wall by Sltb3, a Multidomain Lytic Transglycosylase of Pseudomonas Aeruginosa.
Acs Chem.Biol., 11:1525-, 2016

PubMed Abstract: A family of 11 lytic transglycosylases in Pseudomonas aeruginosa, an opportunistic human pathogen, turn over the polymeric bacterial cell wall in the course of its recycling, repair, and maturation. The functions of these enzymes are not fully understood. We disclose herein that SltB3 of P. aeruginosa is an exolytic lytic transglycosylase. We characterize its reaction and its products by the use of peptidoglycan-based molecules. The enzyme recognizes a minimum of four sugars in its substrate but can process a substrate comprised of a peptidoglycan of 20 sugars. The ultimate product of the reaction is N-acetylglucosamine-1,6-anhydro-N-acetylmuramic acid. The X-ray structure of this enzyme is reported for the first time. The enzyme is comprised of four domains, arranged within an annular conformation. The polymeric linear peptidoglycan substrate threads through the opening of the annulus, as it experiences turnover.

PubMed: 27035839
DOI: 10.1021/ACSCHEMBIO.6B00194

実験手法

X-RAY DIFFRACTION (2.23 Å)