5AHS

3-Sulfinopropionyl-Coenzyme A (3SP-CoA) desulfinase from Advenella mimgardefordensis DPN7T: holo crystal structure with the substrate analog succinyl-CoA

5AHS の概要

• エントリーDOI: 10.2210/pdb5ahs/pdb
• 分子名称: ACYL-COA DEHYDROGENASE, FLAVIN-ADENINE DINUCLEOTIDE, COENZYME A, ... (6 entities in total)
• 機能のキーワード: oxidoreductase
• 由来する生物種: ADVENELLA MIMIGARDEFORDENSIS DPN7
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 270255.60

構造登録者

Cianci, M.,Schuermann, M.,Meijers, R.,Schneider, T.R.,Steinbuechel, A. (登録日: 2015-02-06, 公開日: 2015-06-03, 最終更新日: 2024-01-10)

主引用文献

Schurmann, M.,Meijers, R.,Schneider, T.R.,Steinbuchel, A.,Cianci, M.
3-Sulfinopropionyl-Coenzyme a (3Sp-Coa) Desulfinase from Advenella Mimigardefordensis Dpn7(T): Crystal Structure and Function of a Desulfinase with an Acyl-Coa Dehydrogenase Fold.
Acta Crystallogr.,Sect.D, 71:1360-, 2015

PubMed Abstract: 3-Sulfinopropionyl-coenzyme A (3SP-CoA) desulfinase (AcdDPN7; EC 3.13.1.4) was identified during investigation of the 3,3'-dithiodipropionic acid (DTDP) catabolic pathway in the betaproteobacterium Advenella mimigardefordensis strain DPN7(T). DTDP is an organic disulfide and a precursor for the synthesis of polythioesters (PTEs) in bacteria, and is of interest for biotechnological PTE production. AcdDPN7 catalyzes sulfur abstraction from 3SP-CoA, a key step during the catabolism of DTDP. Here, the crystal structures of apo AcdDPN7 at 1.89 Å resolution and of its complex with the CoA moiety from the substrate analogue succinyl-CoA at 2.30 Å resolution are presented. The apo structure shows that AcdDPN7 belongs to the acyl-CoA dehydrogenase superfamily fold and that it is a tetramer, with each subunit containing one flavin adenine dinucleotide (FAD) molecule. The enzyme does not show any dehydrogenase activity. Dehydrogenase activity would require a catalytic base (Glu or Asp residue) at either position 246 or position 366, where a glutamine and a glycine are instead found, respectively, in this desulfinase. The positioning of CoA in the crystal complex enabled the modelling of a substrate complex containing 3SP-CoA. This indicates that Arg84 is a key residue in the desulfination reaction. An Arg84Lys mutant showed a complete loss of enzymatic activity, suggesting that the guanidinium group of the arginine is essential for desulfination. AcdDPN7 is the first desulfinase with an acyl-CoA dehydrogenase fold to be reported, which underlines the versatility of this enzyme scaffold.

PubMed: 26057676
DOI: 10.1107/S1399004715006616

実験手法

X-RAY DIFFRACTION (2.3 Å)